Effect of 1,5-Anhydro-D-Fructose on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes
Effect of 1,5-Anhydro-D-Fructose on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes
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DOI：
10.1177/1934578x1200701123
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发表时间：
2012-11
影响因子：
1.8
通讯作者：
A. Kojima‐Yuasa;Yohei Deguchi;Y. Konishi;I. Matsui‐yuasa
中科院分区：
文献类型：
--
作者：
A. Kojima‐Yuasa;Yohei Deguchi;Y. Konishi;I. Matsui‐yuasa
1，5-脱水-D-果糖(1，5-AF)是一种结构类似于葡萄糖的单糖。1，5-AF存在于真菌、藻类、大肠杆菌和大鼠肝脏中，由α-1，4-葡聚糖裂解酶催化的淀粉和糖原降解产生。然而，1，5-AF在哺乳动物组织中的生理作用还不是很清楚。在此，我们研究了1，5-AF对3T3-L1脂肪细胞的抗肥胖作用。1，5-AF可显著降低3T3-L1前脂肪细胞和成熟脂肪细胞的GPDH活性而不引起细胞毒性，并通过下调转录因子PPARγ和C/EBPα来抑制细胞内脂质的积累。1，5-AF还可剂量依赖性地诱导细胞能量感受器AMP激活蛋白激酶(AMPK)的磷酸化。而AMPK总蛋白含量无明显变化。此外，1，5-AF增加了3T3-L1脂肪细胞中AMPK激活的重要上游信号--活性氧的水平。我们的结果表明，1，5-AF在体外具有抗肥胖作用，提示1，5-AF可能是一种新型的肥胖和其他代谢性疾病的预防药物。
1,5-Anhydro-D-fructose (1,5-AF) is a monosaccharide that shares a structural similarity to glucose. 1,5-AF is found in fungi, algae, Escherichia coli and rat liver and is produced by the degradation of starch and glycogen, which is catalyzed by the enzyme α-1,4-glucan lyase. However, the physiological role of 1,5-AF in mammalian tissues is not well understood. Here, we investigated the anti-obesity potential of 1,5-AF on adipogenesis in 3T3-L1 adipocytes. 1,5-AF caused a significant decrease in GPDH activity in 3T3-L1 preadipocytes and mature adipocytes without eliciting cytotoxicity, and inhibited cellular lipid accumulation through down-regulation of transcription factors such as PPARγ and C/EBPα. 1,5-AF also induced dose-dependent phosphorylation of AMP-activated protein kinase (AMPK), a cellular energy sensor. However, the total AMPK protein content remained unchanged. Furthermore, 1,5-AF increased the levels of reactive oxygen species, an important upstream signal for AMPK activation in 3T3-L1 adipocytes. Our results show that 1,5-AF exerts anti-obesity action in vitro and suggest that 1,5-AF is potentially a novel preventative agent for obesity and other metabolic diseases.