Gene expression analysis of Estrogenic compounds in the liver of common carp (Cyprinus carpio) using a custom cDNA Microarray
Gene expression analysis of Estrogenic compounds in the liver of common carp (Cyprinus carpio) using a custom cDNA Microarray
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DOI：
10.1002/jbt.20190
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发表时间：
2007-01-01
影响因子：
3.6
通讯作者：
De Coen, W. M.
中科院分区：
文献类型：
--
作者：
Moens, Lotte N.;van der Ven, K.;De Coen, W. M.
暴露于各种具有雌激素活性的化合物已被证明会干扰各种脊椎动物的正常发育和生殖过程。本研究的目的是利用定制的cDNA芯片，确定天然雌激素、17 β -雌二醇和3种合成雌激素化合物（4-壬基酚、双酚A、炔雌醇）在鲤鱼肝脏中的转录谱。为此，将鱼分别暴露在三种浓度的化学物质中24或96小时。微阵列分析显示，在暴露于至少一种雌激素（样）浓度后，总共有185种不同的基因转录物差异表达。我们能够鉴定出一组共有的28个基因片段，它们的表达被三种异种雌激素和17种β -雌二醇以同样的方式显著改变。虽然这些基因表达效应中的一些证实了过去的文献数据，但我们也发现了一些新的（xeno）雌激素暴露的靶基因，为雌激素作用的分子基础提供了有趣的见解。此外，这四种化合物中的每一种都引起了基因表达的变化，而这些变化与其他化学物质不同，或者只是部分不同，这表明并非所有具有雌激素活性的化学物质的作用都是一样的。这些结果证明了我们定制的鲤鱼微阵列在检测常见的雌激素样活性以及识别鱼类中（雌激素）内分泌干扰物的独特化合物相关效应方面的潜力。(c) 2007 Wiley期刊公司
Exposure to a variety of compounds with estrogenic activity has been shown to interfere with normal developmental and reproductive processes in various vertebrate species. The aim of this study was to determine the transcriptional profile of the natural estrogen, 17 beta-estradiol, and three synthetic estrogenic compounds (4-nonylphenol, bisphenol A, ethinylestradiol) in the liver of common carp, using a custom cDNA microarray. For that purpose, fish were aqueously exposed to three concentrations of each chemical for 24 or 96 h. Microarray analysis revealed that a total of 185 different gene transcripts were diff erentially expressed following exposure to at least one of the estrogen(-like) concentrations. We were able to identify a common set of 28 gene fragments, whose expression was significantly modified in the same way by the three xenoestrogens and 17 beta-estradiol. Although several of these gene expression effects corroborated past literature data, we also discovered some novel target genes of (xeno)estrogen exposure, providing interesting insights into the molecular basis of estrogenic effects. In addition, each of the four compounds induced gene expression changes that were not, or only partially, shared by the other chemicals, suggesting that not all chemicals with estrogenic activity act alike. These results demonstrate the potential of our custom Cyprinus carpio microarray to detect common estrogen-like activity as well as to identify unique compound-associated effects of (estrogenic) endocrine disruptors in fish. (c) 2007 Wiley Periodicals, Inc.