THE FUB-1 MUTATION BLOCKS INITIAL MYOFIBRIL FORMATION IN ZEBRAFISH MUSCLE PIONEER CELLS
THE FUB-1 MUTATION BLOCKS INITIAL MYOFIBRIL FORMATION IN ZEBRAFISH MUSCLE PIONEER CELLS
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DOI：
10.1016/0012-1606(91)90314-s
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发表时间：
1991-11-01
影响因子：
2.7
通讯作者：
KIMMEL, CB
中科院分区：
文献类型：
--
作者：
FELSENFELD, AL;CURRY, M;KIMMEL, CB
斑马鱼最早的肌肉是由每个体节中2到6个细胞的重复集合产生的，肌肉先驱（MP）。MP在邻近脊索的年轻躯干肌节中同步发育，正是在那里水平肌隔膜将形成。它们在没有细胞融合的情况下伸长，并且比周围细胞早几个小时分化，从而为肌发生和肌肉图案的体内研究提供了一个简单易行的系统。在MP形成最终的肌原纤维之前，它们组装成长束的含肌动蛋白的细丝，类似于其他人报道的“应力纤维样结构”。Infub-1突变体，所有骨骼肌细胞肌原纤维紊乱，MP出现并正常伸长，但未观察到有序的肌动蛋白丝束。这种缺陷可能是后来的肌原纤维缺陷的基础，这与肌动蛋白丝束对肌肉收缩装置的正确形成至关重要的提议一致。
The earliest muscle in zebrafish arises from iterated sets of two to six cells in each somite, the muscle pioneers (MP). MP develop synchronously in young trunk myotomes adjacent to the notochord, precisely where the horizontal myoseptum will form. They elongate without cell fusion and differentiate hours earlier than surrounding cells, thus providing a simple and accessible system forin vivostudy of myogenesis and muscle patterning. Before the MP form definitive myofibrils they assemble long bundles of actin-containing filaments, similar to “stress-fiber-like structures” reported by others. Infub-1mutants, in which myofibrils are disorganized in all skeletal muscle cells, the MP appear and elongate normally, but ordered actin filament bundles are not seen. This defect could underlie the later myofibrillar ones, consistent with the proposal that actin filament bundles are essential for proper formation of the muscle contractile apparatus.