Size-tunable and biodegradable thrombin-functionalized carboxymethyl chitin microspheres for endovascular embolization
Size-tunable and biodegradable thrombin-functionalized carboxymethyl chitin microspheres for endovascular embolization
复制标题
用于血管内栓塞的尺寸可调且可生物降解的凝血酶功能化羧甲基甲壳素微球
DOI：
10.1016/j.carbpol.2022.119274
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发表时间：
2022
影响因子：
11.2
通讯作者：
Xulin Jiang
中科院分区：
文献类型：
--
作者：
Fan Leng;Sai Lei;Bin Luo;Siyao Lv;Long Huang;Xulin Jiang
血管内栓塞作为一种微创方法，已成为控制出血和治疗肿瘤的有效策略。本文采用不使用交联剂的两水相羧甲基几丁质/聚乙二醇体系制备羧甲基几丁质栓塞微球，共价引入凝血酶制备凝血酶功能化栓塞微球Thr@CMCHm-30。微球尺寸范围为5 ~ 500 μm。体外和体内实验数据表明，这些微球具有良好的可降解性和生物相容性。同时，Thr@CMCHm-30能显著促进血液凝固，增强血凝块强度。更重要的是，Thr@CMCHm-30在大鼠股静脉和兔耳动脉栓塞模型中表现出比市售明胶泡沫Alicon®和聚乙烯醇基栓塞微球CalliSpheres®更好的栓塞效果。因此，可调节大小和可生物降解的凝血酶功能化羧甲基几丁质微球Thr@CMCHm-30在有效止血和血管内栓塞方面具有很大的潜力。
As a minimally invasive method, endovascular embolization has been an effective strategy for controlling bleeding and tumor treatment. Herein, carboxymethyl chitin embolic microspheres were prepared with the aqueous two-phase carboxymethyl chitin/polyethylene glycol system without using any crosslinking agents and thrombin-functionalized embolic microsphere named as Thr@CMCHm-30 was made after covalent introduction of thrombin. The size of the microspheres can be adjusted from 5 to 500 μm. The data of in vitro and in vivo tests indicated that these microspheres possessed good degradability and biocompatibility. Meanwhile, Thr@CMCHm-30 can significantly promote blood clotting and enhance the strength of the blood clots. More importantly, Thr@CMCHm-30 displayed better embolization effect than that of the commercial available Gelfoam Alicon® and polyvinyl alcohol-based embolic microspheres CalliSpheres® in rat femoral vein and rabbit ear artery embolization models. Therefore the size-tunable and biodegradable thrombin-functionalized carboxymethyl chitin microspheres Thr@CMCHm-30 possess great potential for effective hemostasis and endovascular embolization.