Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation
Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation
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DOI：
10.1016/j.jconrel.2006.03.013
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发表时间：
2006-05-30
影响因子：
10.8
通讯作者：
Koyama, Yoshiyuki
中科院分区：
文献类型：
--
作者：
Ito, Tomoko;Iida-Tanaka, Naoko;Koyama, Yoshiyuki
透明质酸（HA）是一种天然的阴离子粘多糖，可以沉积在DNA/聚乙烯亚胺（PEI）复合物的阳离子表面，以重新充电表面电位并减少与蛋白质的非特异性相互作用。HA也可用作靶向特异性细胞受体的配体。此外，HA包被增强了质粒/PEI复合物的转录活性，这可能是通过松开DNA和PEI之间的紧密结合，从而促进转录因子的接近。具有类似于HMG蛋白的结构的具有精胺侧链的两性HA衍生物（Spn-HA）显示出比HA更高的转录增强活性。质粒/PEI/Spn-HA三元复合物在CHO细胞中表现出比裸质粒/PEI复合物高29倍的转基因表达效率。(c)2006 Elsevier B.V保留所有权利。
Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasrnid/PEI complexes in CHO cells. (c) 2006 Elsevier B.V All rights reserved.