Activation of related transforming genes in mouse and human mammary carcinomas.
Activation of related transforming genes in mouse and human mammary carcinomas.
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小鼠和人类乳腺癌中相关转化基因的激活。
DOI：
10.1073/pnas.78.8.5185
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发表时间：
1981
影响因子：
11.1
通讯作者：
Cooper,GM
中科院分区：
文献类型：
--
作者：
Lane,MA;Sainten,A;Cooper,GM
用NIH 3 T3小鼠细胞转染法检测了5个由小鼠乳腺肿瘤病毒（MMTV）诱导的肿瘤、2个由化学致癌物诱导的肿瘤和1个人乳腺肿瘤细胞系（MCF-7）的高分子量DNA中是否存在可传递的活化转化基因。所有五种MMTV诱导的肿瘤、一种化学致癌物诱导的肿瘤和人类肿瘤细胞系的DNA以高效率诱导转化（每微克DNA约0.2 μ g）。NIH细胞转化的MMTV诱导的肿瘤的DNA不包含外源性MMTV DNA序列，表明MMTV诱导的乳腺癌包含激活的细胞转化基因，不连接到病毒DNA。所有五种MMTV诱导的肿瘤、化学致癌物诱导的小鼠肿瘤和人肿瘤细胞系的DNA转化活性通过用限制性内切酶Pvu II和Sac I消化而失活，但不能通过BamHI、EcoRI、HindIII、Kpn I或Xho I消化。这些结果表明，相同或密切相关的转化基因被激活在六个不同的小鼠乳腺癌，诱导由MMTV或化学致癌物，并在人乳腺癌细胞系。
High molecular weight DNAs of five tumors induced by mouse mammary tumor virus (MMTV), two mouse mammary tumors induced by a chemical carcinogen, and one human mammary tumor cell line (MCF-7) were assayed for the presence of transmissible activated transforming genes by transfection of NIH 3T3 mouse cells. DNAs of all five MMTV-induced tumors, one chemical carcinogen-induced tumor, and the human tumor cell line induced transformation with high efficiencies (approximately 0.2 transformant per micrograms of DNA). NIH cells transformed by DNAs of MMTV-induced tumors did not contain exogenous MMTV DNA sequences, indicating that MMTV-induced mammary carcinomas contained activated cellular transforming genes that were not linked to viral DNA. The transforming activities of DNAs of all five MMTV-induced tumors, the chemical carcinogen-induced mouse tumor, and the human tumor cell line were inactivated by digestion with the restriction endonucleases Pvu II and Sac I, but not by BamHI, EcoRI, HindIII, Kpn I, or Xho I. These results indicate that the same or closely related transforming genes were activated in six different mouse mammary carcinomas, induced by either MMTV or a chemical carcinogen, and in a human mammary carcinoma cell line.