The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization
The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization
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DOI：
10.1073/pnas.96.26.14819
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发表时间：
1999-12-21
影响因子：
11.1
通讯作者：
Lutkenhaus, J
中科院分区：
文献类型：
--
作者：
Hu, ZL;Mukherjee, A;Lutkenhaus, J
定位的Z环在大肠杆菌中的中间细胞网站是由min系统，它掩盖了极性网站通过拓扑调节的MinC，分裂抑制剂。为了研究MinC如何抑制分裂，我们产生了保留完整生物活性的MalE-MinC融合体。我们发现，MalE-MinC与FtsZ相互作用，并防止聚合，而不抑制FtsZ的GTP酶活性，MalE-MinC 19抑制分裂的能力降低，对FtsZ的亲和力降低，抑制FtsZ聚合的能力降低。这些结果，沿着MinC定位，表明MinC在细胞的两极之间快速振荡，以使在它们成熟为极性Z环之前形成的FtsZ细丝不稳定。
Positioning of the Z ring at the midcell site in Escherichia coli is assured by the min system, which masks polar sites through topological regulation of MinC, an inhibitor of division. To study how MinC inhibits division, we have generated a MalE-MinC fusion that retains full biological activity. We find that MalE-MinC interacts with FtsZ and prevents polymerization without inhibiting FtsZ's GTPase activity, MalE-MinC19 has reduced ability to inhibit division, reduced affinity for FtsZ, and reduced ability to inhibit FtsZ polymerization. These results, along with MinC localization, suggest that MinC rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings.