CYANINE DYE LABELING REAGENTS CONTAINING ISOTHIOCYANATE GROUPS
CYANINE DYE LABELING REAGENTS CONTAINING ISOTHIOCYANATE GROUPS
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DOI：
10.1002/cyto.990100104
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发表时间：
1989-01-01
期刊：
影响因子：
--
通讯作者：
WAGGONER, AS
中科院分区：
文献类型：
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作者：
MUJUMDAR, RB;ERNST, LA;WAGGONER, AS
合成了新的异硫氰酸酯类菁染料衍生物，作为蛋白质和其他生物分子的荧光共价标记试剂。这些染料在光谱的红色和近红外区域具有最大的吸光度，具有高消光系数和足够的量子产率。在染料结构中加入两个烷基磺酸基增加了其水溶性，这有利于在水溶液中标记生物分子。蛋白质与这些新花青素的反应活性与它们与异硫氰酸荧光素的反应活性相似。这些新的标记试剂是荧光素和罗丹明试剂的补充，扩大了多色分析的可能性。绵羊抗小鼠igg抗体用五甲基菁染料（CY5.8-ITC）标记，并与荧光抗体作为第二试剂，通过流式细胞术检测人t细胞亚群。
New isothiocyanate derivatives of cyanine dyes were synthesized as fluorescent covalent labeling reagents for proteins and other biomolecules. These dyes have maximum absorbance in the red and near infrared regions of the spectrum, have high extinction coefficients and have adequate quantum yields. Incorporating two alkyl sulfonate groups in the dye structures increases their water solubility, which is beneficial for labeling biological molecules in aqueous solution. Reactivities of proteins with these new cyanines are similar to their reactivities with fluorescein isothiocyanate. These new labeling reagents are complementary to the fluorescein and rhodamine reagents, expanding the possibilities of multicolor analyses. Sheep anti-mouse-IgG antibody was labeled with a pentamethine cyanine dye (CY5.8-ITC) and used with a fluoresceinated antibody as a second reagent for detecting human T-cell subsets by flow cytometry.