Novel transcription factors PvBMY1 and PvBMY3 increase biomass yield in greenhouse-grown switchgrass (Panicum virgatum L.)
Novel transcription factors PvBMY1 and PvBMY3 increase biomass yield in greenhouse-grown switchgrass (Panicum virgatum L.)
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DOI：
10.1016/j.plantsci.2018.04.003
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发表时间：
2018-08-01
期刊：
影响因子：
5.2
通讯作者：
Somleva, Maria N.
中科院分区：
文献类型：
--
作者：
Ambavaram, Madana M. R.;Ali, Aminat;Somleva, Maria N.
提高作物产量需要协调光合固碳、中心碳代谢以及最终将碳沉积到最终产物的多种代谢途径。在这项研究中，我们使用了一个基于转录组的基因调控关联网络来寻找转录因子基因，这些基因可以通过与这些过程相关的途径来增加柳枝稷的碳流量，以增加生物量产量。两个新的柳枝稷转录因子，PvBMY 1（生物质产量1，属于APETALA 2/乙烯响应因子家族的转录因子）和PvBMY 3（生物质产量3，核因子Y家族的转录因子的成员），预测的作用，在光合作用和相关代谢的调节。这些基因在柳枝稷中过表达，以确定它们对生物量产量的影响。与野生型对照植物相比，在转基因温室生长的植物中观察到地上生物量和根生物量的显著增加，其中最佳品系产生比对照多160%的地上生物量。鉴定了具有升高的光系统I和II的电子传递速率以及增加的淀粉和可溶性糖水平的转基因株系。
Increasing crop yield requires the coordination of multiple metabolic pathways spanning photosynthetic carbon fixation, central carbon metabolism, and finally targeted carbon deposition to end product. In this study, we used a transcriptome-based gene regulatory association network to search for transcription factor genes that could play a role in increasing carbon flow through pathways associated with these processes to increase biomass yield in switchgrass. Two novel switchgrass transcription factors, PvBMY1 (BioMass Yield 1, belonging to the APETALA2/Ethylene Response Factor family of transcription factors) and PvBMY3 (BioMass Yield 3, a member of the Nuclear-Factor Y family of transcription factors), with predicted roles in the regulation of photosynthesis and related metabolism were identified. These genes were overexpressed in switchgrass to determine their impact on biomass yield. A significant increase in both aboveground and root biomass was observed in transgenic greenhouse grown plants compared to wild-type control plants with the best line producing 160% more aboveground biomass than controls. Transgenic lines with elevated electron transport rate of photosystems I and II as well as increased levels of starch and soluble sugars were identified.