Mutagenic activity in smokeless tobacco products sold in the USA.
Mutagenic activity in smokeless tobacco products sold in the USA.
复制标题
在美国销售的无烟烟草产品的致突变活性。
DOI：
10.1093/carcin/8.5.741
复制
发表时间：
1987
期刊：
影响因子：
4.7
通讯作者：
Guttenplan,JB
中科院分区：
文献类型：
--
作者：
Guttenplan,JB
在美国销售的无烟烟草产品（鼻烟、嚼烟、无烟烟草）的水提取物在碱基对置换突变体鼠伤寒沙门氏菌TA 100中具有致突变性，效力为8000- 16000个回复突变体/g烟草。大部分活性未能提取到有机溶剂中在中性pH和一部分的活性提取到有机溶剂中在酸性pH。烟草特有的亚硝胺，N-亚硝基去甲烟碱和（4-甲基亚硝基氨基）-1-（3-吡啶基）-1-丁酮没有表现出这种行为。诱变需要代谢活化（肝脏S-9组分加NADPH）和弱酸性液体预孵育。诱变行为是典型的亚硝胺，并表明，N-亚硝胺是负责至少在提取物中的一些诱变活性。
Aqueous extracts of smokeless tobacco products (snuff, chewing tobacco, smokeless tobacco) sold in the USA are mutagenic in the base-pair substitution mutant,Salmonella typhimuriumTA100, with potencies of 8000–16 000 revertants/g tobacco. Most of the activity failed to extract into organic solvents at neutral pH and a portion of the activity extracted into organic solvents at acidic pH. The tobacco-specific nitros-amines,N-nitrosonornicotine and (4-methylnitrosamino)-1-(3-pyridyl)-1-butanone did not exhibit this behavior. Mutagenesis required metabolic activation (liver S-9 fraction plus NADPH) and a weakly acidic liquid preincubation. The mutagenesis behavior is typical of nitrosamines, and suggests thatN-nitrosamines are responsible for at least some of the mutagenic activity in the extracts.