CD4-CD8- T-cells in primary Sjogren's syndrome: Association with the extent of glandular involvement
CD4-CD8- T-cells in primary Sjogren's syndrome: Association with the extent of glandular involvement
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DOI：
10.1016/j.jaut.2014.01.030
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发表时间：
2014-06-01
影响因子：
12.8
通讯作者：
Gerli, Roberto
中科院分区：
文献类型：
--
作者：
Alunno, Alessia;Carubbi, Francesco;Gerli, Roberto
目的：越来越多的证据表明，产生IL-17的T细胞，缺乏CD 4和CD 8分子，并被定义为双阴性（DN）细胞，在许多系统性自身免疫性疾病的发病机制中起着关键作用。我们最近证明，这种T细胞亚群在原发性干燥综合征（pSS）患者的外周血（PB）中扩增，产生IL-17并在小唾液腺（MSG）中积累。我们的目的是研究腺和PB DN T细胞在早期pSS，以验证可能的相关性与MSG的组织学模式和临床parameters.Methods：配对的PB单核细胞和MSG从pSS患者的样本进行了评估，在诊断分别通过流式细胞术和免疫荧光染色。组织学分析，以确定组织学评分，BIT细胞分离和存在的生发中心（GC）-like structures也performed.Results：在pSS的早期阶段，循环DN T细胞似乎尚未扩大，并与循环CD 4（+）Th 17细胞呈负相关。浸润ON T细胞的数量与腺体的参与程度，GC样结构和干燥症状的存在，并与循环ON T cells.Conclusions呈负相关：我们的研究结果表明，DN T细胞积极参与的致病机制，导致腺体功能障碍和损伤的pSS，并可能发挥作用，在异位淋巴细胞生成的发展过程中发生的疾病。(C)2014爱思唯尔有限公司版权所有。
Objectives: Growing evidence suggests that IL-17-producing T cells, lacking both CD4 and CD8 molecules and defined as double negative (DN) cells, play a pivotal role in the pathogenesis of a number of systemic autoimmune disorders. We recently demonstrated that this T-cell subset is expanded in the peripheral blood (PB) of patients with primary Sjogren's syndrome (pSS), produces IL-17 and accumulates in minor salivary glands (MSGs). We aimed to investigate glandular and PB DN T cells in early pSS in order to verify a possible correlation with MSGs histological patterns and clinical parameters.Methods: Paired samples of PB mononuclear cells and MSGs from pSS patients were evaluated at the diagnosis by flow cytometry and immunofluorescence staining respectively. Histological analysis to identify histological scores, BIT cell segregation and the presence of germinal center (GC)-like structures was also performed.Results: In early stages of pSS, circulating DN T cells appear to be not yet expanded and inversely correlated with circulating CD4(+)Th17 cells. The number of infiltrating ON T cells were associated with extent of glandular involvement, presence of GC-like structures and dryness symptoms and were inversely correlated with circulating ON T cells.Conclusions: Our findings suggest that DN T cells are actively involved in the pathogenic mechanisms leading to glandular dysfunction and damage in pSS and may play a role in ectopic lymphoneogenesis development occurring during the disease. (C) 2014 Elsevier Ltd. All rights reserved.