Engaging myosin VI tunes motility, morphology and identity in endocytosis.
Engaging myosin VI tunes motility, morphology and identity in endocytosis.
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参与肌球蛋白 VI 调节内吞作用中的运动性、形态和特性。
DOI：
10.1111/tra.12583
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Sivaramakrishnan,Sivaraj
中科院分区：
文献类型：
--
作者：
Ritt,Michael;Sivaramakrishnan,Sivaraj
虽然非常规肌球蛋白与内吞途径的不同阶段相互作用，但它们被归因于控制细胞器身份的蛋白质复合物的次要运输功能。内体受到蛋白质的动态、连续流动的影响，这些蛋白质控制其特征性质，包括其在细胞内的运动性。描述该隔室的身份变化的努力主要集中在隔室上存在的适配器上，而不是隔室本身的运动特性上。在这项研究中，我们使用光遗传学和化学二聚化策略的组合将外源性肌球蛋白VI靶向早期内涵体，并探讨其对细胞器运动，形态和身份的影响。我们跨时间尺度的分析表明了一种模型，其中早期内体上肌球蛋白VI运动性的人工参与限制了基于微管的运动，随后发生以细胞器快速凝聚和解体为特征的形态学变化，最终导致划分内体区室的标记物重叠增强。总之，我们的研究结果表明，肌球蛋白VI运动的合成参与足以改变细胞器内吞途径的稳态。
While unconventional myosins interact with different stages of the endocytic pathway, they are ascribed a transport function that is secondary to the protein complexes that control organelle identity. Endosomes are subject to a dynamic, continuous flux of proteins that control their characteristic properties, including their motility within the cell. Efforts to describe the changes in identity of this compartment have largely focused on the adaptors present on the compartment and not on the motile properties of the compartment itself. In this study, we use a combination of optogenetic and chemical‐dimerization strategies to target exogenous myosin VI to early endosomes, and probe its influence on organelle motility, morphology and identity. Our analysis across timescales suggests a model wherein the artificial engagement of myosin VI motility on early endosomes restricts microtubule‐based motion, followed by morphological changes characterized by the rapid condensation and disintegration of organelles, ultimately leading to the enhanced overlap of markers that demarcate endosomal compartments. Together, our findings show that synthetic engagement of myosin VI motility is sufficient to alter organelle homeostasis in the endocytic pathway.