Enhancing Metabolite Coverage for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Through Multiple On-Tissue Chemical Derivatizations
Enhancing Metabolite Coverage for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Through Multiple On-Tissue Chemical Derivatizations
复制标题
通过多次组织化学衍生化增强基质辅助激光解吸/电离质谱成像的代谢物覆盖范围
DOI：
10.1007/978-1-0716-2030-4_14
复制
发表时间：
2022
影响因子：
--
通讯作者：
Lee, YJ.
中科院分区：
文献类型：
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作者：
O’Neill, K.C.;Dueñas, M.E.;Larson, E.;Forsman, T.T.;Lee, YJ.
在细胞和亚细胞水平上研究和可视化代谢物的能力对于深入了解多细胞生物的生物学途径和代谢非常重要。基质辅助激光解吸/电离（MALDI）质谱成像（MSI）因其高灵敏度和小样本量而成为代谢组学实验的有力分析工具。MALDI-MSI的空间分辨率主要受限于小样本中可用分子的数量。当采样大小低到足以达到细胞或亚细胞的空间分辨率时，信号强度被牺牲，使低电离代谢物难以检测。为了克服这一限制，组织上的化学衍生化反应已被用于通过添加具有永久正电荷或易于电离的官能团来提高选定类别化合物的解吸/电离效率。通过平行利用几种化学衍生化，代谢物覆盖范围可以大大提高。本章概述了使用各种衍生化试剂进行组织化学衍生化的样品制备和数据分析方法。
The ability to study and visualize metabolites on a cellular and sub-cellular level is important for gaining insights into biological pathways and metabolism of multicellular organisms. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is a powerful analytical tool for metabolomics experiments due to its high sensitivity and small sampling size. The spatial resolution in MALDI-MSI is mainly limited by the number of molecules available in a small sampling size. When the sampling size is low enough to achieve cellular or subcellular spatial resolution, signal intensity is sacrificed making poorly ionized metabolites difficult to detect. To overcome this limitation, on-tissue chemical derivatization reactions have been used to enhance the desorption/ionization efficiency of selected classes of compounds by adding a functional group with a permanent positive charge or one that can be easily ionized. By utilizing several chemical derivatizations in parallel, metabolite coverage can be drastically improved. This chapter outlines methodology for sample preparation and data analysis for on-tissue chemical derivatization using various derivatization reagents.