Quantitative determination of SH groups using 19F NMR spectroscopy and disulfide of 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid.
Quantitative determination of SH groups using 19F NMR spectroscopy and disulfide of 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid.
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使用 19F NMR 光谱和 2,3,5,6-四氟-4-巯基苯甲酸的二硫化物定量测定 SH 基团。
DOI：
10.1002/mrc.1652
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
Khramtsov,ValeryV
中科院分区：
文献类型：
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作者：
Potapenko,DmitriiI;Bagryanskaya,ElenaG;Grigoriev,IgorA;Maksimov,AleksanderM;Reznikov,VladimirA;Platonov,VyacheslavE;Clanton,ThomasL;Khramtsov,ValeryV
开发了一种通过 19 F NMR 光谱测量硫醇浓度的新方法。该方法基于硫醇与新合成的氟化二硫化物 2,3,5,6-四氟-4-巯基苯甲酸 (BSSB) 交换反应产物的检测。在硫醇存在下观察到BSSB的 19 F NMR信号显着展宽，并将其归因于母体二硫化物和2,3,5,6-四氟-4-巯基苯甲酸之间的交换反应。发现该反应的速率常数在 pH 7.0 时等于 (63 ± 11) × 103M−1s−1。该方法适用于大鼠血液中谷胱甘肽和白蛋白浓度的测定。版权所有 © 2005 约翰·威利父子有限公司
A new method of measurement of thiol concentration by19F NMR spectroscopy is developed. The method is based on the detection of products of the exchange reaction of thiols with a newly synthesized fluorinated disulfide, 2,3,5,6‐tetrafluoro‐4‐mercaptobenzoic acid (BSSB). A significant broadening of the19F NMR signal of BSSB in the presence of thiols was observed and attributed to the exchange reaction between the parent disulfide and 2,3,5,6‐tetrafluoro‐4‐mercaptobenzoic acid. The rate constant for this reaction was found to be equal to (63 ± 11) × 103M−1s−1at pH 7.0. The method was applied for the measurement of concentration of glutathione and albumin in rat blood. Copyright © 2005 John Wiley & Sons, Ltd.