Effect of protein kinase C activation and Ca2+ mobilization on hexose transport in Swiss 3T3 cells.
Effect of protein kinase C activation and Ca2+ mobilization on hexose transport in Swiss 3T3 cells.
复制标题
蛋白激酶 C 激活和 Ca2+ 动员对 Swiss 3T3 细胞中己糖转运的影响。
DOI：
10.1016/0167-4889(86)90128-x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
A. Iwashima
中科院分区：
文献类型：
--
作者：
K. Kitagawa;H. Nishino;A. Iwashima
通过用佛波醇 12,13-二丁酸酯预处理 24 小时，下调 Ca2+ 激活的磷脂依赖性蛋白激酶（蛋白双酶 C），导致瑞士 3T3 细胞中佛波醇酯诱导的己糖转运活性丧失。然而，在这些细胞中，血小板源性生长因子以及 Ca2+ 离子载体 A23187 仍然能够诱导己糖转运活性的刺激，并伴随细胞内游离 Ca2+ 浓度的升高。由于细胞外 Ca2+ 的螯合抑制了这种刺激，因此细胞外 Ca2+ 流入细胞质似乎对于血小板衍生生长因子和 A23187 对己糖转运的刺激作用至关重要。无论是否存在蛋白激酶C，表皮生长因子和胰岛素也会刺激己糖转运活性。然而，就表皮生长因子而言，发现细胞内Ca2+而非细胞外Ca2+对于刺激是必需的。另一方面，胰岛素刺激己糖转运，不依赖于细胞内和细胞外的 Ca2+。
Down-modulation of Ca2+-activated, phospholipid-dependent protein kinase (protein binase C), which was accomplished by pretreatment with phorbol-12,13-dibutyrate for 24 h, resulted in the loss of a phorbol ester-induced stimulation of hexose transport activity in Swiss 3T3 cells. In these cells, however, platelet-derived growth factor as well as Ca2+ionophore A23187 were still able to induce stimulation of hexose transport activity accompanied by the elevation of intracellular free Ca2+concentration. Since chelation of extracellular Ca2+inhibited this stimulation, inflow of extracellular Ca2+into cytoplasm seemed to be esential for the stimulatory effect of platelet-derived growth factor and A23187 on hexose transport. Epidermal growth factor and insulin also stimulated hexose transport activity regardless of the absence of protein kinase C. However, in the case of epidermal growth factor, intracellular Ca2+, but not extracellular Ca2+, was found to be necessary for the stimulation. On the other hand, insulin stimulated the hexose transport independent of both intra- and extracellular Ca2+.
人多形核白细胞对己糖转运的刺激：蛋白激酶 C 的可能作用。
DOI：
10.1016/0006-291x(85)90626-6
发表时间：
1985
影响因子：
3.1
作者：
McCall,C;Schmitt,J;Cousart,S;O'Flaherty,J;Bass,D;Wykle,R
通讯作者：
Wykle,R