Molecular characterization of the two-component histidine kinase gene from Monilinia fructicola.
Molecular characterization of the two-component histidine kinase gene from Monilinia fructicola.
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DOI：
10.1002/ps.1275
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发表时间：
2006-10
影响因子：
4.1
通讯作者：
Zhonghua Ma;Yong Luo;T. Michailides
中科院分区：
文献类型：
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作者：
Zhonghua Ma;Yong Luo;T. Michailides
核果褐腐病是由核果褐腐病菌Monilinia fructicola（G. Wint）蜂蜜是加州最常见的真菌病之一。本研究采用室内诱变的方法获得了两株耐艾地隆突变株。通过测定渗透压敏感性、对李子的毒力和双组分组氨酸激酶基因（Mfos-1）的序列，研究了果生菌的渗透压敏感性和对李子的毒力。LIR突变体比野生型亲本对渗透胁迫更敏感，对西梅的毒力更低。Mfos-1的氨基酸序列分析表明，该蛋白具有双组分组氨酸激酶基因的所有特征，包括渗透敏感结构域、6个90个氨基酸的重复序列（卷曲螺旋区）、激酶核心和反应调节结构域。LIR突变体和野生型敏感（S）分离株的Mfos-1的DNA序列比较表明，LIR突变体在Mfos-1的卷曲螺旋区有单点突变。
Brown rot of stone fruit caused by Monilinia fructicola (G. Wint) Honey is one of the most common fungal diseases in California. In this study, two laboratory-induced iprodione-resistant (LIR) mutants of M. fructicola were characterized by osmotic sensitivity, virulence on prune and sequence of the two-component histidine kinase gene (Mfos-1). The LIR mutants showed more sensitivity to osmotic stress and lower virulence on prune than their wild-type parent. Analysis of deduced amino acid of Mfos-1 showed that this protein exhibited all the characteristic features of the two-component histidine kinase genes, including osmotic sensing domain, six 90-amino-acid repeat motifs (coiled coil region) and kinase core and response regulator domains. Comparison of DNA sequences of the Mfos-1 from LIR mutants and the wild-type sensitive (S) isolate showed that LIR mutants had single point mutations in the coiled coil region of Mfos-1.