Organic acid profiling in adipocyte differentiation of 3T3-F442A cells: increased production of Krebs cycle acid metabolites.
Organic acid profiling in adipocyte differentiation of 3T3-F442A cells: increased production of Krebs cycle acid metabolites.
复制标题
3T3-F442A 细胞脂肪细胞分化中的有机酸分析：克雷布斯循环酸代谢物的产生增加。
DOI：
10.1016/0026-0495(92)90216-w
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Goldman,P
中科院分区：
文献类型：
--
作者：
Lai,RK;Goldman,P
为了寻找脂肪组织的特殊代谢特征，我们检测了在胰岛素和胎牛血清的影响下，3T3-F442a小鼠成纤维细胞向脂肪细胞分化时释放到培养液中的有机酸，每隔48小时改变一次。我们鉴定了13种不同的有机酸，其中4种(苹果酸、富马酸、琥珀酸和2-羟基戊二酸)在18天的分化期间从3倍增加到10倍。分化发生后，从培养液中删除胰岛素会导致苹果酸、富马酸和2-羟基戊二酸的浓度略有下降。其他酸没有受到影响，包括18种我们无法用气相色谱-质谱仪鉴定的酸。没有发现酸只存在于成纤维细胞或脂肪细胞的培养液中。
To try to find special metabolic characteristics of adipose tissue, we examined the organic acids released into the culture medium, which was changed every 48 hours, as 3T3-F442A mouse fibroblasts underwent differentiation to adipocytes under the influence of insulin and fetal calf serum. We identified 13 different organic acids, of which four (malate, fumarate, succinate, and 2-hydroxyglutarate) increased from threefold to 10-fold during an 18-day period of differentiation. After differentiation had occurred, the deletion of insulin from the culture medium resulted in a slight decrease in the concentration of malate, fumarate, and 2-hydroxyglutarate. Other acids were not affected, including 18 that we were unable to identify by gas chromatographymass spectroscopy. No acid was found to be present only in the medium of either fibroblasts or adipocytes.