SERS quantitative analysis of trace ferritin based on immunoreaction regulation of graphene oxide catalytic nanogold reaction
SERS quantitative analysis of trace ferritin based on immunoreaction regulation of graphene oxide catalytic nanogold reaction
复制标题
基于氧化石墨烯催化纳米金反应免疫反应调控的微量铁蛋白SERS定量分析
DOI：
10.1016/j.snb.2018.02.080
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发表时间：
2018-06-15
影响因子：
8.4
通讯作者：
Jiang, Zhiliang
中科院分区：
文献类型：
--
作者：
Li, Chongning;Liu, Yuyao;Jiang, Zhiliang
在50 ℃条件下，HAuCl 4-H2 O2的金纳米反应非常缓慢，氧化石墨烯（GO）对该反应表现出很强的催化作用，在分子探针维多利亚蓝（VB 4 r）存在下，金纳米颗粒（AuNPs）在1614 cm（-1）处具有很强的表面增强拉曼散射（Sers）活性。随着GO含量的增加，金纳米粒子的生成量增加，Sers强度也随之增加。铁蛋白抗体（Ab）可以与GO表面结合以形成Ab-GO，其可以抑制纳米催化。当铁蛋白（Ag）存在时，产生的Ab-Ag免疫复合物从GO表面分离，这导致GO催化的恢复。铁蛋白浓度在0.017-1.02 ng/mL范围内与增强的Sers信号Δ I_（1614）cm ~（-1）呈线性关系。据此，建立了一种基于调节GO纳米催化作用的铁蛋白Sers定量分析新方法。(C)2018 Elsevier B. V.版权所有。
In 50 degrees C conditions, the gold nanoreaction of HAuCl4-H2O2 is very slow, graphene oxide (GO) exhibited strong catalysis on the nanoreaction to form gold nanoparticles (AuNPs) with strong surface-enhanced Raman scattering (SERS) activity at 1614 cm(-1) in the presence of molecular probes of Victoria Blue (VB4r). The SERS intensity increased with the GO increasing due to formation of more AuNPs. The ferritin antibody (Ab) can bind to GO surface to form Ab-GO that can inhibit the nanocatalysis. When ferritin (Ag) existed, the produced immunocomplexes of Ab-Ag are separated from the GO surface, which led to the recovery of GO catalysis. The enhanced SERS signal Delta I1614cm-1 is linear to ferritin concentration in the range of 0.017-1.02 ng/mL. Accordingly, a new SERS quantitative analysis method was established for detection of ferritin, based on the regulating GO nanocatalysis. (C) 2018 Elsevier B.V. All rights reserved.