A new acylated oleanane triterpenoid from Couepia polyandra that inhibits the lyase activity of DNA polymerase beta.
A new acylated oleanane triterpenoid from Couepia polyandra that inhibits the lyase activity of DNA polymerase beta.
复制标题
一种来自 Couepia Polyandra 的新型酰化齐墩果烷三萜类化合物，可抑制 DNA 聚合酶 β 的裂解酶活性。
DOI：
10.1021/np0301893
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Kingston,DavidGI
中科院分区：
文献类型：
--
作者：
PrakashChaturvedula,VS;Gao,Zhijie;Hecht,SidneyM;Jones,ShannonH;Kingston,DavidGI
采用生物测定法对Couepiapolyandra的正己烷提取物进行分级分离，检测DNA聚合酶β裂解酶活性的抑制剂，结果分离出新的三萜3β，16 β，23-三乙酰氧基齐墩果酸-12-烯-28-酸（1）和四种已知化合物，油酸，白桦酸，豆甾醇和β-谷甾醇。新化合物的结构是在广泛的1D和2D NMR光谱解释的基础上建立的。所有五种化合物均抑制DNA聚合酶β裂解酶活性。
Bioassay-directed fractionation of an-hexane extract ofCouepiapolyandrausing an assay to detect inhibitors of the lyase activity of DNA polymerase β resulted in the isolation of the new triterpene 3β,16β,23-triacetoxyolean-12-en-28-oic acid (1) and four known compounds, oleanolic acid, betulinic acid, stigmasterol, and β-sitosterol. The structure of the new compound was established on the basis of extensive 1D and 2D NMR spectroscopic interpretation. All five compounds inhibited DNA polymerase β lyase activity.