Expression of parathyroid hormone-related protein mRNA in the rat before birth: demonstration by hybridization histochemistry.
Expression of parathyroid hormone-related protein mRNA in the rat before birth: demonstration by hybridization histochemistry.
复制标题
出生前大鼠甲状旁腺激素相关蛋白 mRNA 的表达：杂交组织化学证实。
DOI：
--
复制
发表时间：
1991
影响因子：
3.5
通讯作者：
F. Beck
中科院分区：
文献类型：
--
作者：
P. Senior;D. Heath;F. Beck
应用杂交组织化学方法研究了大鼠胚胎着床至足月不同时期甲状旁腺激素（PTH）及其相关蛋白（PTHrP）基因表达的个体发生。PTHrP mRNA在着床后早期滋养层巨细胞中表达，但在13.5天前从该部位消失。通过原位技术检测到的局部基因表达开始于胚胎组织的12.5至15.5天。PTHrP基因表达的分布提示PTHrP可能参与着床过程。其在胚胎和胎儿组织中广泛分布，但明显定位，与旁分泌或自分泌功能一致，可能与转化生长因子-β家族的生长因子有关。甲状旁腺激素的表达只发生在甲状旁腺和胎儿甲状旁腺在妊娠13.5天检测。
The ontogeny of parathyroid hormone (PTH) and PTH-related protein (PTHrP) gene expression was studied by hybridization histochemistry in the rat at various stages between implantation and full term. PTHrP mRNA was demonstrable in the early postimplantation trophoblastic giant cells but disappeared from this site before 13.5 days. Localized gene expression, detectable by the in-situ technique, began between 12.5 and 15.5 days in embryonic tissues. The distribution of gene expression suggests that PTHrP may be concerned with the process of implantation. Its widespread, yet clearly localized, distribution in embryonic and fetal tissues is consistent with a paracrine or autocrine function which may relate to the transforming growth factor-beta family of growth factors. PTH expression occurred solely in the parathyroid and was detectable in the fetal parathyroid at 13.5 days of gestation.