Cellular resolution circuit mapping with temporal-focused excitation of soma targeted channelrhodopsin
Cellular resolution circuit mapping with temporal-focused excitation of soma targeted channelrhodopsin
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DOI：
10.7554/elife.14193
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发表时间：
2016-08-15
期刊：
影响因子：
7.7
通讯作者：
Bolton, M. McLean
中科院分区：
文献类型：
--
作者：
Baker, Christopher A.;Elyadat, Yishai M.;Bolton, M. McLean
我们描述了电路映射的光遗传学方法的改进，使功能性突触连接与单神经元分辨率的测量成为可能。通过扩大双光子束在成像平面使用时间聚焦方法和限制通道视紫红质的索马和近端树突，我们能够可靠地唤起动作电位在个别神经元，验证尖峰生成与GCaMP6，并确定存在或不存在的突触连接与膜片钳电生理记录。
We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single-neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proximal dendrites, we are able to reliably evoke action potentials in individual neurons, verify spike generation with GCaMP6s, and determine the presence or absence of synaptic connections with patch-clamp electrophysiological recording.