Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin‐like protein Hkr1 but not Msb2
Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin‐like protein Hkr1 but not Msb2
复制标题
酶解酶对酵母细胞壁完整性 MAPK 通路的激活取决于蛋白酶和葡聚糖酶的活性，并且需要粘蛋白样蛋白 Hkr1 而不是 Msb2
DOI：
10.1016/j.febslet.2013.09.030
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发表时间：
2013
期刊：
影响因子：
3.5
通讯作者：
J. Arroyo
中科院分区：
文献类型：
--
作者：
J. M. Rodríguez;Sonia Díez;Clara Bermejo;C. Nombela;J. Arroyo
酵母对细胞壁完整性受损的条件的适应主要依赖于细胞壁完整性（CWI）丝裂原激活蛋白激酶（MAPK）途径。酶解酶是一种细胞壁消化酶的混合物，可触发一种特殊的信号传导机制，其中 CWI 途径的激活依赖于高渗透性甘油 MAPK 途径。我们已经鉴定了酶解酶中主要酶活性的抑制剂，并测试了它们对 CWI 途径 MAPK（Slt2/Mpk1）激活的影响。最终，只有 β-1,3-葡聚糖酶和蛋白酶活性对于引发 Slt2 激活并赋予酶解酶裂解能力至关重要。此外，我们还发现渗透传感器 Hkr1 是信号传导所必需的，它是迄今为止发现的最上游元件。
Yeast adaptation to conditions in which cell wall integrity is compromised mainly relies on the cell wall integrity (CWI) mitogen-activated protein kinase (MAPK) pathway. Zymolyase, a mixture of cell wall-digesting enzymes, triggers a peculiar signaling mechanism in which activation of the CWI pathway is dependent on the high-osmolarity glycerol MAPK pathway. We have identified inhibitors of the principal enzyme activities present in zymolyase and tested their effect on the activation of the MAPK of the CWI pathway, Slt2/Mpk1. Eventually, only β-1,3-glucanase and protease activities were essential to elicit Slt2 activation and confer lytic power to zymolyase. Moreover, we show that the osmosensor Hkr1 is required for signaling, being the most upstream element identified to date.