Cu2+ modulated silver nanoclusters as an on–off–on fluorescence probe for the selective detection of L-histidine
Cu2+ modulated silver nanoclusters as an on–off–on fluorescence probe for the selective detection of L-histidine
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Cu(2) 调制的银纳米簇作为开关荧光探针，用于选择性检测 L-组氨酸。
DOI：
10.1016/j.bios.2014.11.013
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发表时间：
2015
影响因子：
12.6
通讯作者：
石硕
中科院分区：
文献类型：
--
作者：
郑絮月;姚天明;朱影;石硕
在本研究中，开发了一种基于 Cu2+ 介导的 DNA 模板银纳米簇 (DNA-Ag NCs) 的新策略，作为无标记、开-关-开荧光探针，用于检测 l-组氨酸。对八种合成的 DNA 寡核苷酸 (D1–D8) 进行了实验测试，最终选择 D5-Ag NCs 由于其最佳的荧光特性用于 l-组氨酸检测。 D5-Ag NCs 的荧光发射可以通过电子或能量转移被 Cu2+ 猝灭。添加 1-组氨酸后，Cu 2+ 与 1-组氨酸的咪唑基团之间的螯合导致 Cu 2+ 从 D5-Ag NC 中释放，随后导致探针的荧光显着增强。与所有其他氨基酸相比，该方法对组氨酸具有良好的选择性，在 0.20–80 μM 范围内呈线性关系，检测限 (LOD) 为 4.3 nM。该策略还成功应用于检测稀释的人尿液中的l-组氨酸，在生物系统中展现出巨大的实际应用机会。
In the present study, a new strategy based on Cu2+mediated DNA-templated silver nanoclusters (DNA-Ag NCs) was developed, as a label-free, on–off–on fluorescent probe for the detection ofl-histidine. Eight synthesized DNA oligonucleotides (D1–D8) were experimentally tested, and D5-Ag NCs was finally selected forl-histidine detection due to its best fluorescent properties. The fluorescence emission of D5-Ag NCs could be quenched by Cu2+via electron or energy transfer. Upon addition ofl-histidine, the chelation between Cu2+and the imidazole group ofl-histidine leads to Cu2+liberation from D5-Ag NCs, and subsequently results in a dramatic fluorescence enhancement of the probe. The method displayed a good selectivity towardl-histidine over all the other amino acids, with a linear relationship in the range of 0.20–80 μM, and a limit of detection (LOD) of 4.3 nM. The strategy was also successfully applied to detectl-histidine in diluted human urine, exhibiting great opportunities for practical application in biological system.