Increased Expression of Fibrillin‐1 in Human Corneas with Bullous Keratopathy
Increased Expression of Fibrillin‐1 in Human Corneas with Bullous Keratopathy
复制标题
患有大疱性角膜病的人角膜中 Fibrillin-1 的表达增加
DOI：
10.1097/00003226-199805000-00012
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发表时间：
1998
期刊：
影响因子：
2.8
通讯作者：
M. Kenney
中科院分区：
文献类型：
--
作者：
A. Ljubimov;M. Saghizadeh;K. Spirin;R. Mecham;L. Sakai;M. Kenney
目的研究人大泡性角膜病变（PBK/ABK）角膜组织中微纤维蛋白的表达。方法对正常和PBK/ABK角膜进行免疫荧光染色，以检测角膜基质蛋白-1和-2。采用半定量逆转录-聚合酶链反应（RT-PCR）和Southern杂交技术检测细胞中β-内酰胺酶-1（β-内酰胺酶-1）mRNA的表达。结果正常角膜和病变角膜中仅检测到β-内酰胺酶-1。如前所述，在正常角膜中，在角膜缘基质和基底膜（BM）中以及在利姆布斯附近的短距离的周边角膜上皮BM中发现。中央角膜BM、基质和后弹力层均为阴性。所有的PBK/ABK角膜均为白细胞介素-1阳性，其在后弹力层内皮面、上皮BM、上皮下纤维化区域和后胶原层的纤维沉积物中检测到。RT-PCR检测到正常角膜中低水平的Bclin-1 mRNA，而在PBK/ABK角膜中Bclin-1 mRNA显著增加。结论PBK/ABK角膜组织中的tenascin-1和tenascin-C的表达可能是术后角膜水肿形成大疱和后胶原层的异常纤维化/伤口愈合过程的一部分。
Purpose To characterize the expression of fibrillins, microfibril components, in human corneas with pseudophakic/aphakic (PBK/ABK) bullous keratopathy. Methods Normal and PBK/ABK corneas were stained by immunofluorescence for fibrillin-1 and −2. The expression of fibrillin-1 messenger RNA (mRNA) was studied by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and Southern analysis. Results Only fibrillin-1 was detected in normal and diseased corneas. As described previously, in normal corneas, it was found in the limbal stroma and basement membrane (BM) and in the peripheral corneal epithelial BM for a short distance near the limbus. Central corneal BM, stroma, and Descemet's membrane were negative. All PBK/ABK corneas were positive for fibrillin-1, which was detected in fibrillar deposits at the endothelial face of Descemet's membrane, in the epithelial BM, subepithelial fibrosis areas, and posterior collagenous layer. By RT-PCR, low levels of fibrillin-1 mRNA were detected in normal corneas, and they increased significantly in PBK/ABK corneas. Conclusion The deposition of fibrillin-1, together with tenascin-C, in PBK/ABK corneas may be part of an abnormal fibrotic/wound-healing process that occurs during the development of postsurgical corneal edema with the formation of bullae and posterior collagenous layer.