Immunolocalization of a novel collectin CL-K1 in murine tissues
Immunolocalization of a novel collectin CL-K1 in murine tissues
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DOI：
10.1369/jhc.7a7312.2007
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发表时间：
2008-03-01
影响因子：
3.2
通讯作者：
Wakamiya, Nobutaka
中科院分区：
文献类型：
--
作者：
Motomura, Wataru;Yoshizaki, Takayuki;Wakamiya, Nobutaka
我们最近发现了一种新的聚集蛋白，CL-K1，它可能在先天免疫中发挥作用，作为聚集蛋白家族的一员。在这项使用小鼠的研究中，我们研究了CL-K1的组织分布，以更好地了解其病理生理学相关性。实时荧光定量PCR分析表明，CL-K1 mRNA在所有测试的组织中表达。免疫组化分析表明，CL-K1表达在近端肾小管的肾脏，胃肠道粘膜，和细支气管的支气管腺体中的SP-A和SP-D在这些肺结构中的定位相似。免疫组织化学还显示，CL-K1在中央周围的肝细胞中高度表达，这表明鼠CL-K1可能主要在肝和肝静脉中产生，像人CL-K1一样分泌到血流中。CL-K1在几种组织中的血管平滑肌中尤其被检测到。此外，它还在肠潘氏细胞、肾系膜细胞、胰岛D细胞和脑神经元中表达。令人感兴趣的是，CL-K1的这种表达谱在胶原凝集素中是独特的。这些组织学结果可能有助于了解这种新型聚集蛋白的生物学功能。
We have recently identified a novel collectin, CL-K1, that may play a role in innate immunity as a member of the collectin family. In this study using mice, we investigated the tissue distribution of CL-K1 for better understanding of its pathophysiological relevance. Real-time PCR analyses demonstrated that CL-K1 mRNA was expressed in all tissues tested. Immunohistochemical analyses demonstrated that CL-K1 was expressed in proximal tubules of kidney, in mucosa of the gastrointestinal tract, and in bronchial glands of bronchioles similar to the localization of SP-A and SP-D in these pulmonary structures. Immunohistochemistry also showed that CL-K1 was highly expressed in hepatocytes around the central which suggests that murine CL-K1 may be mainly produced in the liver and veins in liver, secreted into the blood stream as is human CL-K1. CL-K1 was especially detected in vascular smooth muscle in several types of tissues. In addition, it was also expressed in intestinal Paneth cells, in mesangial cells of kidney, in pancreatic islet D cells, and in neurons of the brain. It is of interest that this profile of CL-K1 expression is unique among the collectins. Together these histological findings may be useful for understanding the biological function of this novel collectin.