Preparative separation of vitexin and isovitexin from pigeonpea extracts with macroporous resins
Preparative separation of vitexin and isovitexin from pigeonpea extracts with macroporous resins
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DOI：
10.1016/j.chroma.2006.11.015
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发表时间：
2007-01-19
影响因子：
4.1
通讯作者：
Tong, Meihong
中科院分区：
文献类型：
--
作者：
Fu, Yujie;Zu, Yuangang;Tong, Meihong
牡荆素和异牡荆素是木豆叶中的主要成分，具有多种药理活性。本研究采用大孔吸附树脂对牡荆素和异牡荆素进行了分离纯化。对ADS-5、ADS-7、ADS-8、ADS- 11、ADS- 17、ADS-21、ADS-31和ADS-F8 8种大孔吸附树脂的性能和吸附特性进行了评价。研究结果表明，ADS-5型大孔吸附树脂最适合于牡荆素和异牡荆素的分离。采用Langmuir和Freundlich等温线描述了不同温度下溶质与树脂之间的相互作用，平衡实验数据与两条等温线拟合良好。采用ADS-5树脂填充柱进行动态吸附和解吸试验，优化分离工艺。最佳吸附条件为：上样液中牡荆素和异牡荆素的浓度分别为0.22和0.40 mg/mL，处理体积为3 BV，流速为1 mL/min，pH值为4，温度为25 ℃，洗脱剂为乙醇-水（40：60，v/v），5 BV作为洗脱液，流速：经ADS-5树脂处理一次后，牡荆素和异牡荆素的含量分别从0.86%提高了4.07倍和11.52倍，1.53%~ 3.50%和17.63%，回收率分别为65.03%和73.99%。因此，ADS-5树脂吸附-解吸法可简便、有效地实现牡荆素和异牡荆素的制备分离，该方法可为其他植物中黄酮C-糖苷的分离提供参考。(c)2006 Elsevier B. V.保留所有权利。
Vitexin and isovitexin are a pair of isomeric compounds known as the major constituents in pigeonpea leaves and possess various pharmacological activities. In the present study, the preparative separation of vitexin and isovitexin with macroporous resins (Nankai Hecheng S & T, Tianjin, China) was studied. The performance and adsorption characteristics of eight macroporous resins including ADS-5, ADS-7, ADS-8, ADS- 11, ADS- 17, ADS-21, ADS-31 and ADS-F8 have been evaluated. The research results indicate that ADS-5 resin is most appropriate for the separation of vitexin and isovitexin. Langmuir and Freundlich isotherms were used to describe the interactions between solutes and resin at different temperatures, and the equilibrium experimental data were well fitted to the two isotherms. Column packed with ADS-5 resin was used to perform dynamic adsorption and desorption tests to optimize the separation process. The optimum parameters for adsorption were as follows: the concentration of vitexin and isovitexin in sample solution: 0.22 and 0.40 mg/mL, respectively, processing volume: 3 BV, flow rate: I mL/min, pH 4, temperature: 25 degrees C; for desorption: ethanol-water (40:60, v/v), 5 BV as an eluent, flow rate: I mL/min. After one run treatment with ADS-5 resin, the contents of vitexin and isovitexin were increased 4.07-fold and 11.52-fold from 0.86%, 1.53% to 3.50% and 17.63%, the recovery yields were 65.03% and 73.99%, respectively. In conclusion, the preparative separation of vitexin and isovitexin can be easily and effectively achieved via adsorption and desorption on ADS-5 resin, and the method can be referenced for the separation of other flavone C-glucosides from herbal materials. (c) 2006 Elsevier B.V. All rights reserved.