Isolation of Methyl Parathion-Degrading Strain M6 and Cloning of the Methyl Parathion Hydrolase Gene
Isolation of Methyl Parathion-Degrading Strain M6 and Cloning of the Methyl Parathion Hydrolase Gene
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DOI：
10.1128/aem.67.10.4922-4925.2001
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发表时间：
2001-10
影响因子：
4.4
通讯作者：
Z. Cui;Shun-Peng Li;Guoping Fu
中科院分区：
文献类型：
--
作者：
Z. Cui;Shun-Peng Li;Guoping Fu
摘要分离到一株降解甲基对硫磷的细菌M6，初步鉴定为邻单胞菌属（Plesiomonaspp.）。利用鸟枪法克隆技术从基因组文库中筛选到一个新的有机磷水解酶基因mpd。测定了mpd基因的核苷酸序列。该基因在大肠杆菌中得到了高效表达。
ABSTRACT A degradative bacterium, M6, was isolated and presumptively identified as Plesiomonas sp. strain M6 was able to hydrolyze methyl parathion to p-nitrophenol. A novel organophosphate hydrolase gene designated mpd was selected from its genomic library prepared by shotgun cloning. The nucleotide sequence of the mpd gene was determined. The gene could be effectively expressed in Esherichia coli.