Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration
Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration
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DOI：
10.1016/j.toxlet.2006.12.001
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发表时间：
2007-01-30
影响因子：
3.5
通讯作者：
Chai, Zhifang
中科院分区：
文献类型：
--
作者：
Wang, Jiangxue;Zhou, Guoqiang;Chai, Zhifang
为评价纳米二氧化钛颗粒的毒性，以超细二氧化钛颗粒(155 Nm)为对照，研究了纳米二氧化钛颗粒(25 nm和80 nm)对成年小鼠的急性毒性。由于毒性低，按照OECD的程序，固定大剂量的5g/kg体重的二氧化钛混悬液按OECD程序一次性经口灌胃。在2周内，二氧化钛颗粒没有明显的急性毒性。然而，在纳米(25和80 nm)组中，雌性小鼠的肝脏系数很高。血清生化指标(ALT/AST、LDH)和肝脏病理(中心静脉周围水变性、肝细胞点状坏死)的变化表明，大量不同大小的二氧化钛颗粒暴露后可引起肝损伤。此外，还观察到各试验组的肾毒性，如BUN水平升高和肾脏病理改变。25 nm和80 nm照射组血清LDH和α-HBDH较对照组有明显变化，提示心肌损伤。但心、肺、睾丸(卵巢)和脾组织未见异常病理改变。生物分布实验表明，二氧化钛主要滞留在肝、脾、肾、肺等组织中，说明二氧化钛颗粒经胃肠道摄取后可转运到其他组织器官。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
In order to evaluate the toxicity of TiO2 particles, the acute toxicity of nano-sized TiO2 particles (25 and 80 nm) on adult mice was investigated compared with fine TiO2 particles (155 nm). Due to the low toxicity, a fixed large dose of 5 g/kg body weight of TiO2 suspensions was administrated by a single oral gavage according to the OECD procedure. In 2 weeks, TiO2 particles showed no obvious acute toxicity. However, the female mice showed high coefficients of liver in the nano-sized (25 and 80 nm) groups. The changes of serum biochemical parameters (ALT/AST, LDH) and pathology (hydropic degeneration around the central vein and the spotty necrosis of hepatocytes) of liver indicated that the hepatic injury was induced after exposure to mass different-sized TiO2 particles. In addition, the nephrotoxicity like increased BUN level and pathology change of kidneys was also observed in the experimental groups. The significant change of serum LDH and alpha-HBDH in 25 and 80 nm groups showed the myocardial damage compared with the control group. However, there are no abnormal pathology changes in the heart, lung, testicle (ovary), and spleen tissues. Biodistribution experiment showed that TiO2 mainly retained in the liver, spleen, kidneys, and lung tissues, which indicated that TiO2 particles could be transported to other tissues and organs after uptake by gastrointestinal tract. (c) 2006 Elsevier Ireland Ltd. All rights reserved.