Estradiol inhibits ATP-induced intracellular calcium concentration increase in dorsal root ganglia neurons
Estradiol inhibits ATP-induced intracellular calcium concentration increase in dorsal root ganglia neurons
复制标题
DOI：
10.1016/s0306-4522(02)00915-6
复制
发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Micevych, PE
中科院分区：
文献类型：
--
作者：
Chaban, VV;Mayer, EA;Micevych, PE
雌激素被认为与痛觉的调节有关。虽然这种调节发生在中枢神经系统，但雌激素也可能作用于细胞体位于背根神经节(DRG)内的初级传入神经元。用Fura-2负载原代培养的大鼠背根神经节神经元，通过荧光比值成像检测ATP诱导的细胞内钙离子浓度([Ca~(2+)](I))的变化。ATP是一种镇痛剂，通过激活嘌呤能2X(P2X)型受体和电压门控钙通道(VGCC)而引起[Ca~(2+)](I)的变化。在53%的中小背根节神经元上，三磷酸腺苷(10微米)引起[Ca~(2+)](I)瞬变(226.6.+/-16.7 nM，n=42)增加。17β-雌二醇(100 NM)孵育5分钟可抑制ATP诱导的[Ca~(2+)](164+/-14.6 nM，P
Estrogen has been implicated in modulation of pain processing. Although this modulation occurs within the CNS, estrogen may also act on primary afferent neurons whose cell bodies are located within the dorsal root ganglia (DRG). Primary cultures of rat DRG neurons were loaded with Fura-2 and tested for ATP-induced changes in intracellular calcium concentration ([Ca2+](i)) by fluorescent ratio imaging. ATP, an algesic agent, induces [Ca2+](i) changes via activation of purinergic 2X (P2X) type receptors and voltage-gated Ca2+ channels (VGCC). ATP (10 muM) caused increased [Ca2+](i) transients (226.6.+/-16.7 nM, n = 42) in 53% of small to medium DRG neurons. A 5-min incubation with 17beta-estradiol (100 nM) inhibited ATP-induced [Ca2+](i) (164+/-14.6 nM, P