A little VEGF goes a long way. Therapeutic angiogenesis by direct injection of vascular endothelial growth factor-encoding plasmid DNA.
A little VEGF goes a long way. Therapeutic angiogenesis by direct injection of vascular endothelial growth factor-encoding plasmid DNA.
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一点点 VEGF 就大有帮助。
DOI：
10.1161/01.cir.94.12.3062
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发表时间：
1996
期刊：
影响因子：
37.8
通讯作者：
Majesky,MW
中科院分区：
文献类型：
--
作者：
Majesky,MW
局部产生少量的对内皮细胞具有内在特异性的强效分泌型生长因子可以绕过对高效基因转移载体和精心设计的治疗方案的需要，从而在缺血性外周血管疾病中产生有效的治疗性血管生成。这是Tsurumi等人在本期Circulation 1中发表的报告中的信息，其中编码165个氨基酸分泌形式的人血管内皮生长因子（hVEGF-165）的裸质粒DNA通过直接肌内注射引入缺血的兔后肢。与仅接受质粒DNA注射的对照后肢相比，在缺血后肢肌肉内的五个位点中的每一个处注射100 μg hVEGF-165质粒DNA后30天，可以证明侧支血管形成增加、血流改善和毛细血管轮廓数目增加。本研究中描述的基因转移方法的简单性表明，对于血管内血管成形术导管为基础的基因递送方法不可取或不可能的广泛外周血管疾病患者，这是一种潜在有用的替代方法。
Local production of small amounts of a potent, secreted growth factor with built-in specificity for endothelial cells may bypass the need for highly efficient gene transfer vectors and elaborate treatment protocols to produce effective therapeutic angiogenesis in ischemic peripheral vascular disease. That is the message of a report by Tsurumi et al that appears in this issue of Circulation 1 in which naked plasmid DNA encoding the 165–amino acid secreted form of human vascular endothelial growth factor (hVEGF-165) is introduced by direct intramuscular injection into ischemic rabbit hindlimbs. Thirty days after injection of 100 μg hVEGF-165 plasmid DNA at each of five sites within ischemic hindlimb muscles, increased collateral vessel formation, improved blood flow, and an increased number of capillary profiles could be demonstrated compared with control hindlimbs that received injections of plasmid DNA only. The simplicity of the gene transfer approach described in this study suggests a potentially useful alternative for patients with extensive peripheral vascular disease in whom intravascular angioplasty catheter–based gene delivery methods are inadvisable or not possible.