Fertilization channels in ascidian eggs are not activated by Ca.
Fertilization channels in ascidian eggs are not activated by Ca.
复制标题
海鞘卵中的受精通道不被 Ca 激活。
DOI：
10.1016/0014-4827(87)90405-8
复制
发表时间：
1987
影响因子：
3.7
通讯作者：
Dale,B
中科院分区：
文献类型：
--
作者：
Dale,B
Using the whole-cell voltage clamp technique, experiments were carried out on ascidian eggs to determine the role of intracellular Ca in the gating of fertilization channels. Raising the level of Ca by adding Ca to the intracellular perfusion medium or by loading the egg cortex (> 50 μM) with Ca through voltage gated channels did not lead to the activation of fertilization channels. Alternatively, eggs exposed to low-Ca seawater, perfused with the chelator K-EGTA or Ca channel blocking agents to prevent the release of Ca from intracellular organelles, and subsequently inseminated generated fertilization currents. This argues against Ca as a second messenger in the activation of fertilization channels in the ascidian egg and alternative mechanisms are discussed.
