Junctional Adhesion Molecules (JAMs) are differentially expressed in fibroblasts and co-localize with ZO-1 to adherens-like junctions
Junctional Adhesion Molecules (JAMs) are differentially expressed in fibroblasts and co-localize with ZO-1 to adherens-like junctions
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DOI：
10.1080/15419060600877978
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发表时间：
2006-07-01
影响因子：
--
通讯作者：
Cunningham, Sonia A.
中科院分区：
文献类型：
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作者：
Morris, Andrew P.;Tawil, Ahmad;Cunningham, Sonia A.
连接黏附分子(JAMs)是上皮细胞和内皮细胞紧密连接的组成部分和调节器。由于天然成纤维细胞粘附样连接的分子组成仍未得到很好的描述，我们确定了JAM在成纤维细胞中的表达谱。我们在人的皮肤、肺和角膜原代成纤维细胞培养上发现了JAM-C。在小鼠体内，JAM-A存在于L细胞，JAM-C存在于3T3L1细胞，JAM-A和JAM-C在NIH3T3成纤维细胞中共表达。在原代真皮成纤维细胞中，JAM-C集中在相对细胞之间形成的拉链状连接。双重免疫染色显示JAM-C与ZO-1细胞内支架分子在细胞接触处共定位，长度从7微米到超过25微米不等。JAM-C还标记了N-钙粘附素和钙粘附素-11抗体检测到的类似拉链状连接。我们的结论是，内源性JAM-C是真皮成纤维细胞粘附样连接的一个组成部分，我们的研究结果将JAM的表达和潜在功能扩展到了间质组织。
Junctional Adhesion Molecules (JAMs) are components and regulators of the well-characterized epithelial and endothelial tight junction. Since the molecular components of native fibroblast adherens-like junctions remain poorly described we determined JAM expression profiles in fibroblasts. We found JAM-C on human dermal, lung, and corneal primary fibroblast cultures. Within murine lines, JAM-A was found in L-cells, JAM-C in 3T3 L1 cells, and both JAM-A and JAM-C were co-expressed in NIH 3T3 fibroblasts. In primary dermal fibroblasts, JAM-C concentrated at zipper-like junctions that formed between apposing cells. Dual immunostaining showed JAM-C co-localization with the ZO-1 intracellular scaffolding molecule at cell contacts that ranged from 7 mu m to over 25 mu m in length. JAM-C also labeled similar zipper-like junctions detected with N-Cadherin and Cadherin-11 antibodies. We conclude that endogenous JAM-C is an integral component of the dermal fibroblast adherens-like junction, and our data extend the expression and potential function of JAMs into mesenchymal tissues.