Repeat microsphere delivery for serial measurement of regional blood perfusion in the chronically instrumented, conscious canine
Repeat microsphere delivery for serial measurement of regional blood perfusion in the chronically instrumented, conscious canine
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DOI：
10.1016/j.jss.2007.04.012
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发表时间：
2008-03-01
影响因子：
2.2
通讯作者：
Godleski, John J.
中科院分区：
文献类型：
--
作者：
Bartoli, Carlo R.;Okabe, Kazunori;Godleski, John J.
导论.对于慢性，在清醒的狗重复血流动力学研究，我们设计和测试了一个长期仪表犬微球输送模型。本研究的目的是（1）研究使用荧光标记微球重复估计血液灌注的准确性，以及（2）开发和验证一种长期制剂，该制剂允许在相同的清醒动物中进行连续估计。通过胸廓切开术，9只犬的左心耳和主动脉血管通路导管连接到皮下血管通路端口。在11或18周的过程中，四只动物接受了160万个15 gm微球的七次连续注射（总计：1120万个），五只动物接受了225万个微球的八次连续注射（总计：1800万个）。所有导管在方案期间保持双向通畅14.9 +/- 0.8（平均值+/- SEM）周。在任一组动物中，球体积聚均未显著改变整体心肌（P = 0.69，P = 0.25）、肾脏（P = 0.92，P = 0.12）、肝脏（P = 0.84，P = 0.32）或脾脏（P = 0.33，P = 0.70）血液灌注。导管保持双向通畅数月，不干扰制备的血流动力学反应，并允许重复经皮注射微球和从清醒犬体内抽取参考动脉血。在18周内连续注射8次，总计1800万个微球，未改变局部心肌、肝、肾或脾血流。这种可靠的、慢性的、经皮动脉通路准备提供了一种方法，用于检查病理生理学、药物和环境影响对清醒的大型动物局部动脉血流的急性和长期影响。(c)2008年爱思唯尔公司All rights reserved.
Introduction. For chronic, repeat hemodynamic studies in conscious dogs, we designed and tested a chronically instrumented canine microsphere delivery model. The goals of this study were (1) to investigate the accuracy of repeated estimations of blood perfusion using fluorescent-labeled microspheres and (2) to develop and validate a chronic preparation that permits consecutive estimations in the same conscious animal over an extended protocol.Methods. Via thoracotomy, nine dogs were instrumented with left atrial appendage and aortic vascular access catheters connected to subcutaneous vascular access ports. Four animals received seven serial injections of 1.6 million 15 gm microspheres (total: 11.2 million), and five animals received 8 serial injections of 2.25 million microspheres (total: 18 million) over the course of 11 or 18 wk.Results. All catheters have remained bidirectionally patent during protocol for 14.9 +/- 0.8 (mean +/- SEM) wk. Sphere accumulation did not significantly alter global myocardial (P = 0.69, P = 0.25), renal (P = 0.92, P = 0.12), hepatic (P = 0.84, P = 0.32), or splenic (P = 0.33, P = 0.70) blood perfusion in either set of animals.Conclusions. Catheters remained bidirectionally patent for months, did not interfere with the hemodynamic responses of the preparation, and allowed repeat percutaneous injection of microspheres and withdrawal of reference arterial blood from within conscious canines. Eight serial injections totaling 18 million microspheres over 18 weeks did not alter regional myocardial, hepatic, renal, or splenic blood flow. This dependable, chronic, percutaneous arterial access preparation provides a means for examining acute and long-term effects of pathophysiological, pharmaceutical, and environmental influences on regional arterial blood flow in conscious, large animals. (c) 2008 Elsevier Inc. All rights reserved.