Time-dependent hormetic effects of 1-alkyl-3-methylimidazolium bromide on Vibrio qinghaiensis sp.-Q67: Luminescence, redox reactants and antioxidases
Time-dependent hormetic effects of 1-alkyl-3-methylimidazolium bromide on Vibrio qinghaiensis sp.-Q67: Luminescence, redox reactants and antioxidases
复制标题
1-烷基-3-甲基溴化咪唑鎓对青海弧菌-Q67的时间依赖性毒物动态效应：发光、氧化还原反应物和抗氧化酶
DOI：
10.1016/j.chemosphere.2012.11.070
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发表时间：
2013-04-01
期刊：
影响因子：
8.8
通讯作者：
Zhang, Jin
中科院分区：
文献类型：
--
作者：
Zhang, Jing;Liu, Shu-Shen;Zhang, Jin
由于越来越多的证据表明离子液体（IL）的毒性，其绿色证书正受到挑战。毒物兴奋效应进一步引起了他们对生态的关注。然而，对于毒物兴奋效应的时间依赖性变化及其机制的研究仍然很少。在本研究中，我们研究了四种1-烷基-3-甲基溴化咪唑鎓([amim]Br)，包括1-乙基([emim]Br)、-丁基([bmim]Br)、-己基([hmim]Br)和-辛基([omim]Br)对青海弧菌-Q67发光的时间依赖性毒物动态效应。结果表明，侧链较短的[amim]Br、[emim]Br和[bmim]Br引起明显的毒物动态时间依赖性毒性。 [emim]Br 和 [bmim]Br 毒效效应的有效浓度 (EC) 值随着时间的推移而增加。具有较长侧链的[amim]Br、[hmim]Br和[omim]Br对发光产生S形浓度依赖性抑制，并且EC50值几乎不变。为了说明这一机制，我们随后检查了氧化还原反应物和抗氧化酶的反应。 [emim]Br和[bmim]Br显着诱导FMN（黄素单核苷酸）、NADH（还原型烟酰胺腺嘌呤二核苷酸）、SOD（超氧化物歧化酶）和CAT（过氧化氢酶），并且诱导作用随着时间的推移而增加，这与它们对Q67的毒效作用随时间的变化相似。同时，[hmim]Br和[omim]Br对氧化还原反应物和抗氧化酶没有造成显着影响。总之，[amim]Br 对发光、氧化还原反应物和抗氧化酶的毒物效应表现出对暴露时间和侧链的依赖性。我们的研究结果为了解 [emim]Br 和 [bmim]Br 对发光细菌及其生化指标的毒物兴奋效应的时间依赖性生物过程提供了见解。 (C) 2012 年，爱思唯尔有限公司出版。
The green credentials of ionic liquids (ILs) are being challenged due to the increasing evidence of their toxicity. The hormetic effects further raised their ecological concern. However, it remained poorly studied on the time-dependent changes of the hormetic effects and the mechanisms. In this study, we investigated the time-dependent hormetic effects of four 1-alkyl-3-methylimidazolium bromide ([amim]Br), including 1-ethyl ([emim]Br), -butyl ([bmim]Br), -hexyl ([hmim]Br) and -octyl ([omim]Br), on the luminescence of Vibrio qinghaiensis sp.-Q67. The results showed that [amim]Br with shorter side chains, [emim]Br and [bmim]Br, caused obvious hormetic time-dependent toxicities. The effective concentration (EC) values for the hormetic effects of [emim]Br and [bmim]Br increased with time. [amim]Br with longer side chains, [hmim]Br and [omim]Br, produced sigmoid concentration-dependent inhibitions on the luminescence, and the EC50 values almost unchanged. To illustrate the mechanism, we subsequently examined the responses of redox reactants and antioxidases. [emim]Br and [bmim]Br significantly induced FMN (flavin mononucleotide), NADH (reduced nicotinamide adenine dinucleotide), SOD (superoxide dismutase) and CAT (catalase), and the inductions increased with time, which is similar to the time-dependent changes of their hormetic effects on Q67. Meanwhile, [hmim]Br and [omim]Br did not cause significant effects on the redox reactants and antioxidases. In conclusion, the hormetic effects of [amim]Br on the luminescence, redox reactants and antioxidases showed the dependence on both exposure time and side chains. Our findings provided insights into the time-dependent biological process of the hormetic effects of [emim]Br and [bmim]Br on the photobacterium and its biochemical indicators. (C) 2012 Published by Elsevier Ltd.