STRUCTURE ELUCIDATION OF GLYCOSPHINGOLIPIDS AND GANGLIOSIDES USING HIGH-PERFORMANCE TANDEM MASS-SPECTROMETRY
STRUCTURE ELUCIDATION OF GLYCOSPHINGOLIPIDS AND GANGLIOSIDES USING HIGH-PERFORMANCE TANDEM MASS-SPECTROMETRY
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DOI：
10.1021/bi00405a021
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发表时间：
1988-03-08
期刊：
影响因子：
2.9
通讯作者：
COSTELLO, CE
中科院分区：
文献类型：
--
作者：
DOMON, B;COSTELLO, CE
使用快原子轰击高性能串联质谱 (FABMS/MS) 对鞘糖脂和神经节苷脂进行了研究。已经研究了同源化合物以确定碎片。正离子模式下分子种类的碰撞诱导解离光谱主要提供有关神经酰胺构成的信息（糖苷配基作为整个N-酰基残基和长链碱基），而负离子光谱则显示有关糖序列和碳水化合物支化程度的片段。对于携带复杂寡糖部分的神经节苷脂，已经进行了碎片离子的碰撞光谱以获得额外的结构数据。串联质谱法相对于传统快原子轰击质谱法 (FABMS) 的优势在于，可以记录混合物中各个组分的碰撞光谱，就像此类样品中通常遇到的那样。此外，从基质中排除大部分干扰信号可以识别低质量的相关片段。
Glycosphingolipids and gangliosides have been investigated by using fast atom bombardment high-performance tandem mass spectrometry (FABMS/MS). Homologous compounds have been investigated in order to ascertain the fragmentation. Collision-induced dissociation spectra of the molecular species in the positive ion mode mainly afford information on the ceramide constitution (aglycon as a whole N-acyl residue, and long-chain base), whereas negative ion spectra show fragments informative of the sugar sequence and the degree of branching of the carbohydrate. In the case of gangliosides carrying a complex oligosaccharide moiety, collision spectra of fragment ions have been performed in order to gain additional structural data. The advantage of tandem mass spectrometry over conventional fast atom bombardment mass spectrometry (FABMS) consists in the fact that collision spectra of the individual components from mixtures, as usually encountered with these kinds of samples, can be recorded. Furthermore, the exclusion of most of the interferring signals from the matrix allows the identification of pertinent fragments at low mass.