Comprehensive analysis of the functional TCR repertoire at the single-cell level
Comprehensive analysis of the functional TCR repertoire at the single-cell level
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DOI：
10.1016/j.bbrc.2008.01.011
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发表时间：
2008-03-21
影响因子：
3.1
通讯作者：
Muraguchi, Atsushi
中科院分区：
文献类型：
--
作者：
Ozawa, Tatsuhiko;Tajiri, Kazuto;Muraguchi, Atsushi
分析V β TCR库以了解免疫应答中的t细胞群。然而，由于没有合适的分析方法，很难分析V α -V β对的TCR库。在这里，我们应用单细胞5'-RACE方法从单个t细胞中扩增TCR cdna，并分析了对超抗原SEB有反应的人t细胞中的V α -V β对。我们发现seb刺激的CD4(+) t细胞的TCR V β谱与之前的报道一致，TCR V α谱也表现出显著的差异，并且seb应答的t细胞的TCR V α -V β对是混杂的。我们还在单个t细胞中发现了显著的双TCR α表达。这是在单个t细胞水平上对V α -V β对功能库进行全面分析的第一份报告。这种新方法可能有助于基于tcr的免疫治疗。(c) 2008爱思唯尔公司版权所有。
A V beta TCR repertoire is analyzed for understanding the T-cell population in the immune response. However, the TCR repertoire of the V alpha-V beta pair is difficult to analyze because no suitable analytical method is available. Here, we have applied the single-cell 5'-RACE method for amplifying TCR cDNAs from single T-cells and analyzed the repertoire of V alpha-V beta pairs in human T-cells that responded to a superantigen, SEB. We found that the TCR V beta profile of the SEB-stimulated CD4(+) T-cells was in accordance with the previous reports, that the TCR V alpha profile also exhibited a prominent difference, and that the TCR V alpha-V beta pairs of the SEB-responding T-cells were promiscuous. We have also found a significant dual TCR alpha expression in single T-cells. This is the first report of a comprehensive analysis of the functional repertoire of V alpha-V beta pairs at the single T-cell level. This novel method may contribute to TCR-based immunotherapeutics. (c) 2008 Elsevier Inc. All rights reserved.