DIFFERENTIAL EXPRESSION AND RELEASE OF CD54 INDUCED BY CYTOKINES
DIFFERENTIAL EXPRESSION AND RELEASE OF CD54 INDUCED BY CYTOKINES
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DOI：
10.1002/hep.1840220326
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发表时间：
1995-09-01
期刊：
影响因子：
13.5
通讯作者：
SMITH, CW
中科院分区：
文献类型：
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作者：
MICKELSON, JK;KUKIELKA, G;SMITH, CW
细胞间粘附分子-1 （ICAM-1, CD54）在受细胞因子刺激的许多细胞类型中上调，人肝母细胞瘤细胞系（C3A， HepG2/C3的亚克隆，目前被用作替代肝脏）和人肺腺癌细胞（A549）受到白细胞介素-1 β (IL-1 β)、肿瘤坏死因子α (TNF α)、干扰素γ (IFN γ)、或IL-6，以确定细胞类型对ICAM-1上调的个体细胞因子的反应性差异。每个细胞因子在细胞培养基中评估ICAM-1 mRNA，表面表达和cICAM-1，在3至6小时之间，IL-1 β (30 U/mL)刺激肝细胞ICAM-1 mRNA的最大增加，其次是IFN γ (100 U/mL), TNF α (30 U/mL)和IL-6 （100 U/mL）。除IL-6外，细胞因子诱导的肝细胞表面ICAM-1水平（免疫荧光细胞术，mAb R6.5）呈剂量依赖性，浓度越高，ICAM-1水平受到抑制。干扰素γ刺激后水平最高（P < 0.05）。IL-1 β和TNF α均明显减少；IL-6 (P
Intercellular adhesion molecule-1 (ICAM-1, CD54) is upregulated in many cell types stimulated by cytokines, A human hepatoblastoma cell line (C3A, a subclone of HepG2/C3 that is currently being used as a surrogate liver) and human lung adenocarcinoma cells (A549) were stimulated with interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF alpha), interferon-gamma (IFN gamma), or IL-6 to determine any differences in cell type responsiveness to individual cytokines for ICAM-1 upregulation. Time courses were performed with each cytokine evaluating ICAM-1 mRNA, surface expression, and cICAM-1 in the cell culture media, Between 3 and 6 hours, IL-1 beta (30 U/mL) stimulated the greatest increase in hepatocyte ICAM-1 mRNA, followed by IFN gamma (100 U/mL), TNF alpha (30 U/mL), and IL-6 (100 U/mL) in order of potency. Except for IL-6, cytokine-induced hepatocyte surface levels of ICAM-1 (immunofluorescence now cytometry, mAb R6.5) were dose dependent, with inhibition at higher concentration. Highest levels followed stimulation with IFN gamma (P < .05). Significantly less was found after both IL-1 beta and TNF alpha; none was detected after IL-6 (P