Cytotoxic Screening of Medicinal and Edible Plants in Okinawa, Japan, and Identification of the Main Toxic Constituent of Rhodea japonica (Omoto)
Cytotoxic Screening of Medicinal and Edible Plants in Okinawa, Japan, and Identification of the Main Toxic Constituent of Rhodea japonica (Omoto)
复制标题
日本冲绳药食同源植物的细胞毒性筛选及Rhodea japonica（Omoto）主要有毒成分的鉴定
DOI：
10.1271/bbb.67.1401
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Akira Nonaka
中科院分区：
文献类型：
--
作者：
T. Masuda;Y. Oyama;Natsuko Yamamoto;C. Umebayashi;H. Nakao;Y. Toi;Y. Takeda;Katsuo Nakamoto;Hideki Kuninaga;Y. Nishizato;Akira Nonaka
使用K562人白血病细胞，通过流式细胞术测定了冲绳栽培的36种药用和食用植物的53个部分的乙醇提取物的细胞毒活性。两种来自Rhodea japonica 和Hypericum chinense 的提取物在浓度为10 μg/ml 时具有细胞毒性。分离并鉴定出Rhodea japonica 的主要细胞毒性成分为Rhodexin A，它已被分离为该植物的强心剂。 Rhodexin A 对 K562 细胞生长的 IC50 值为 19 nM，该活性比哇巴因 (IC50, 60 nM) 强得多。
The cytotoxic activity of ethanol extracts from 53 parts of 36 species of medicinal and edible plants cultivated in Okinawa was measured by using K562 human leukemia cells by a flow cytometric method. Two extracts from Rhodea japonica and Hypericum chinense were cytotoxic at a concentration of 10 μg/ml. The main cytotoxic constituent of Rhodea japonica was isolated and identified to be rhodexin A, which has been isolated as a cardetonic agent of the plant. The IC50 value for rhodexin A against the growth of K562 cells was 19 nM, this activity being much stronger than that of ouabain (IC50, 60 nM).