Elongation in translation as a dynamic interaction among the ribosome, tRNA, and elongation factors EF-G and EF-Tu.
Elongation in translation as a dynamic interaction among the ribosome, tRNA, and elongation factors EF-G and EF-Tu.
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DOI：
10.1017/s0033583509990060
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发表时间：
2009-08
影响因子：
6.1
通讯作者：
Frank, Joachim
中科院分区：
文献类型：
--
作者：
Agirrezabala, Xabier;Frank, Joachim
核糖体是一个复杂的大分子机器，它翻译信使RNA中编码的信息，并通过连接由同源转移RNA（tRNA）携带的单个氨基酸来合成多肽。蛋白质的延伸周期，在此期间，tRNA以协调的方式沿着沿着超过100 μ m的路径穿过核糖体，这是由核糖体的大规模重排促进的。这些重排与tRNA的构象变化以及分别催化tRNA递送和易位的延伸因子EF-Tu和EF-G -GTP酶密切相关。这篇评论的重点是与核糖体机制的动力学，这是基础，结合现有的生化，动力学和荧光共振能量转移数据，我们的知识的解码和易位步骤的蛋白质延伸的结构数据。
The ribosome is a complex macromolecular machine that translates the message encoded in the messenger RNA and synthesizes polypeptides by linking the individual amino acids carried by the cognate transfer RNAs (tRNAs). The protein elongation cycle, during which the tRNAs traverse the ribosome in a coordinated manner along a path of more than 100 Å, is facilitated by large-scale rearrangements of the ribosome. These rearrangements go hand in hand with conformational changes of tRNA as well as elongation factors EF-Tu and EF-G – GTPases that catalyze tRNA delivery and translocation, respectively. This review focuses on the structural data related to the dynamics of the ribosomal machinery, which are the basis, in conjunction with existing biochemical, kinetic, and fluorescence resonance energy transfer data, of our knowledge of the decoding and translocation steps of protein elongation.