Single base-resolution methylome of the silkworm reveals a sparse epigenomic map
Single base-resolution methylome of the silkworm reveals a sparse epigenomic map
复制标题
蚕的单碱基分辨率甲基化组揭示了稀疏的表观基因组图谱
DOI：
10.1038/nbt.1626
复制
发表时间：
2010-05-01
影响因子：
46.9
通讯作者：
Wang, Jun
中科院分区：
文献类型：
--
作者：
Xiang, Hui;Zhu, Jingde;Wang, Jun
昆虫的表观遗传调控可能对多种生物学过程产生影响。在这里，我们调查的模式昆虫，家蚕的甲基化组，在单碱基分辨率使用Illumina高通量亚硫酸氢盐测序（MethylC-Seq）。我们保守估计，基因组胞嘧啶的0.11%是甲基胞嘧啶，所有这些都可能发生在CG二核苷酸。CG甲基化在基因体中大量富集，并且与基因表达水平正相关，表明其在基因转录中具有积极作用。我们发现，转座因子，启动子和核糖体DNA低甲基化，但相比之下，基因体中的小RNA匹配的基因组位点是密集甲基化。这项工作有助于我们理解昆虫的表观遗传学，与先前对拟南芥（1）和人类（2）高度甲基化基因组的研究相反，展示了一种对生物体（如具有低水平甲基化的昆虫）表观基因组进行测序的策略。
Epigenetic regulation in insects may have effects on diverse biological processes. Here we survey the methylome of a model insect, the silkworm Bombyx mori, at single-base resolution using Illumina high-throughput bisulfite sequencing (MethylC-Seq). We conservatively estimate that 0.11% of genomic cytosines are methylcytosines, all of which probably occur in CG dinucleotides. CG methylation is substantially enriched in gene bodies and is positively correlated with gene expression levels, suggesting it has a positive role in gene transcription. We find that transposable elements, promoters and ribosomal DNAs are hypomethylated, but in contrast, genomic loci matching small RNAs in gene bodies are densely methylated. This work contributes to our understanding of epigenetics in insects, and in contrast to previous studies of the highly methylated genomes of Arabidopsis(1) and human(2), demonstrates a strategy for sequencing the epigenomes of organisms such as insects that have low levels of methylation.