Chemiluminescent cholesterol sensor based on peroxidase-like activity of cupric oxide nanoparticles
Chemiluminescent cholesterol sensor based on peroxidase-like activity of cupric oxide nanoparticles
复制标题
基于氧化铜纳米颗粒的过氧化物酶样活性的化学发光胆固醇传感器
DOI：
10.1016/j.bios.2012.11.031
复制
发表时间：
2013-05-15
影响因子：
12.6
通讯作者：
Lin, Xin-Hua
中科院分区：
文献类型：
--
作者：
Hong, Lei;Liu, Ai-Lin;Lin, Xin-Hua
A chemiluminescent cholesterol sensor with good selectivity and enhanced sensitivity was constructed based upon the peroxidase-like activity of cupric oxide nanoparticles. Cupric oxide nanoparticles can catalyze the oxidation of luminol by H2O2, which was produced by the reaction of cholesterol and oxygen that was catalyzed by cholesterol oxidase. Therefore, the oxidation of cholesterol could be transduced into the chemiluminescence of luminol by combining these two reactions. Under the optimum conditions, the CL intensity was proportional to the concentration of cholesterol over the range of 0.625-12.5 mu M and a detection limit was 0.17 mu M. The applicability of proposed method has been validated by determination of cholesterol in milk powder and human serum samples with satisfactory results. (c) 2012 Elsevier B.V. All rights reserved.
