Assessing the protective effects of cryptotanshinone on CoCl(2)‑induced hypoxia in RPE cells.
Assessing the protective effects of cryptotanshinone on CoCl(2)‑induced hypoxia in RPE cells.
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DOI：
10.3892/mmr.2021.12379
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发表时间：
2021-10
影响因子：
3.4
通讯作者：
Lu P
中科院分区：
文献类型：
--
作者：
Gu Y;Liu W;Liu G;Li X;Lu P
几种视网膜疾病的发生与缺氧密切相关。隐丹参酮（cryptotanshinone，CT）作为中药丹参的有效成分，其对缺氧条件下视网膜细胞的影响尚不清楚。本研究旨在探讨CT对氯化钴（CoCl 2）诱导的缺氧条件下视网膜色素上皮（RPE）细胞的保护作用。采用细胞计数试剂盒-8法、Annexin V-FITC/PI染色、逆转录-定量PCR和蛋白质印迹法检测CT对ARPE-19细胞的作用。CT（10和20 µM）降低了CoCl 2诱导的ARPE-19细胞中血管内皮生长因子表达和缺氧诱导转录因子-1 α表达的增加。另外，CT通过调节Bcl-2和Bax蛋白表达减轻缺氧诱导的细胞凋亡。CT处理还降低了CoCl 2诱导的IL-6、IL-1β和TNF-α mRNA水平的升高。总之，CT可以保护RPE细胞在CoCl 2诱导的缺氧中免于凋亡和炎症，并且这些结果保证了其作为治疗缺氧性眼病的药物的价值的进一步体内研究。
The development of several retinal diseases is closely related to hypoxia. As a component of the Traditional Chinese medicine Salvia miltiorrhiza, the effects of cryptotanshinone (CT) on retinal cells under hypoxic conditions are not well understood. The aim of the present study was to explore how CT exerted its protective effects on retinal pigment epithelium (RPE) cells under hypoxic conditions induced by cobalt chloride (CoCl2). The effects of CT were investigated using a Cell Counting Kit-8 assay, Annexin V-FITC/PI staining, reverse transcription-quantitative PCR and western blotting in ARPE-19 cells. CT (10 and 20 µM) reduced the CoCl2-induced increase in vascular endothelial growth factor expression and hypoxia-inducible transcription factor-1α expression in ARPE-19 cells. Additionally, CT alleviated hypoxia-induced apoptosis by regulating Bcl-2 and Bax protein expression. CT treatment also reduced the increase in the mRNA levels of IL-6, IL-1β and TNF-α induced by CoCl2. In summary, CT may protect RPE cells against apoptosis and inflammation in CoCl2-induced hypoxia, and these results warrant further in vivo study into its value as a drug for treating hypoxic eye diseases.