Variation of glucosinolates in 62 varieties of Chinese cabbage (Brassica rapa L. ssp pekinensis) and their antioxidant activity
Variation of glucosinolates in 62 varieties of Chinese cabbage (Brassica rapa L. ssp pekinensis) and their antioxidant activity
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DOI：
10.1016/j.lwt.2014.03.001
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发表时间：
2014-09-01
影响因子：
6
通讯作者：
Kim, Sun-Ju
中科院分区：
文献类型：
--
作者：
Lee, Min-Ki;Chun, Jin-Hyuk;Kim, Sun-Ju
对62个大白菜品种的硫代葡萄糖苷（GSL）和抗氧化活性进行了研究。通过HPLC和DPPH、HRSA和FRAP测定来测定。五种脂肪族GSL：鉴定了前瓜依春、黑芥子苷、葡糖阿利辛、乌藨子苷和乌藨子苷;四种吲哚基GSL：4-羟基乌藨子苷、乌藨子苷、4-甲氧基乌藨子苷和新乌藨子苷;一种芳香族GSL：乌藨子苷。Glucobrassicanapin和glucoprassicanapin记录最丰富（平均4.52和3.72 μ mol/g DW，分别）。62个品种的总GSL含量差异较大（2.83 ~ 48.53 μ mol/g DW）。不同品种间GSL总含量和个别含量也存在较大差异。吲哚基和芳香基GSL共占GSL总量的26%，但品种间差异不大。FC 7和FI 17可能是未来育种计划的良好候选者，因为它们具有高含量的glucobrassicin（分别为2.10和1.66 μ mol/g DW）。与阳性对照相比，大部分白菜品种表现出明显的抗氧化活性。然而，三种抗氧化剂测定与总GSL无显著相关性。在某些品种中存在大量的glucobrassicin应进行更广泛的研究，因为GSL是吲哚-3-甲醇，一种有效的抗癌异硫氰酸酯的前体。(C)2014爱思唯尔有限公司版权所有。
Glucosinolate (GSL) and antioxidant activity in 62 varieties of Chinese cabbage (Brassica rapa L ssp. pekinensis) were determined by HPLC and DPPH, HRSA, and FRAP assays. Five aliphatic GSLs: progoitrin, sinigrin, glucoalyssin, gluconapin, and glucobrassicanapin; four indolyl GSLs: 4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin; one aromatic GSL: gluconasturtiin were identified. Glucobrassicanapin and gluconapin documented the most abundant (average 4.52 and 3.72 mu mol/g DW, respectively). The contents of total GSLs varied extensively among 62 varieties (range from 2.83 to 48.53 mu mol/g DW). Comprehensive differences in total and individual GSL contents have also been observed among different varieties. Indolyl and aromatic GSL together accounted 26% of the total GSLs; but there are few differences among varieties. FC7 and FI17 could be good candidates for future breeding programs since they had a high quantity of glucobrassicin (2.10 and 1.66 mu mol/g DW, respectively). Most of the Chinese cabbage varieties showed significant antioxidant activities when compare with positive control. However, three antioxidant assays were not significantly correlated with total GSLs. The presence of significant quantities of glucobrassicin in some varieties should be studied more extensively, since GSL is the precursor of indole-3-carbinol, a potent anticancer isothiocyanate. (C) 2014 Elsevier Ltd. All rights reserved.