Microbial diversity in hydrothermal surface to subsurface environments of Suiyo Seamount, Izu-Bonin Arc, using a catheter-type in situ growth chamber
Microbial diversity in hydrothermal surface to subsurface environments of Suiyo Seamount, Izu-Bonin Arc, using a catheter-type in situ growth chamber
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DOI：
10.1016/s0168-6496(04)00004-2
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发表时间：
2004-03-15
影响因子：
4.2
通讯作者：
Maruyama, A
中科院分区：
文献类型：
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作者：
Higashi, Y;Sunamura, M;Maruyama, A
在伊豆-小笠原岛弧水阳海隆深海热液喷口附近使用便携式海底钻探机进行挖掘后，使用称为喷口导管的原位生长室对从钻孔内采集的样本进行了微生物多样性检查。该仪器，我们设计的这项研究，包括一个耐热管顶端的钛网截留胶囊，包装与灭菌的无机多孔颗粒，这是作为一个粘附基板。将该仪器在每个钻孔内以及天然喷口内部署后，在热喷口流体（最高温度：156- 305 ℃）附近持续3-10天，从粘附颗粒中提取DNA，扩增16 S rDNA，并随机选择克隆进行测序。在超过120个克隆的系统发育分析中，在ε-Proteobacteria，光合细菌（PSB）相关的α-Proteobacteria和Euryarchaeota集群内检测到几种新的细菌类型。经常遇到ε-变形菌门的成员。其中一半被分为两个已知的组，Corre的B和D。另一半克隆被分配到新的组，SSSV-BE 1和SSSV-BE 2（水予海山次喷口起源，细菌域，ε-变形菌门，组1和2）。从这个热液喷口领域，我们发现了一个新的谱系内的PSB集群，SSNV-BAI（水予海山自然喷口的起源，细菌域，α-变形菌，组1），这是密切相关的Rhodopila globiformis分离的温泉。从钻孔样品中还发现了一些古细菌克隆。这些克隆形成了一个新的单系进化枝，SSSV-AE 1（水阳Scamount亚喷口起源，Euryarchaeota，第1组），大约在产甲烷超嗜热成员之间的Methanococcales和环境克隆成员的DHVE组II。因此，这种热液喷口环境似乎是一种值得注意的微生物和遗传资源。还值得注意的是，这里提出的一些发现是通过将原位生长室应用于钻孔深处的热流体而实现的。(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
After excavation using a portable submarine driller near deep-sea hydrothermal vents in the Suiyo Seamount, Izu-Bonin Arc, microbial diversity was examined in samples collected from inside the boreholes using an in situ growth chamber called a vent catheter. This instrument, which we devised for this study, consists of a heat-tolerant pipe tipped with a titanium mesh entrapment capsule that is packed with sterilized inorganic porous grains, which serve as an adhesion substrate. After this instrument was deployed inside each of the boreholes, as well as a natural vent, for 3-10 days in the vicinity of hot vent fluids (maxima: 156-305degreesC), DNA was extracted from the adhesion grains, 16S rDNA was amplified, and randomly selected clones were sequenced. In phylogenetic analysis of more than 120 clones, several novel phylotypes were detected within the epsilon-Proteobacteria, photosynthetic bacteria (PSB)-related alpha-Proteobacteria, and Euryarchaeota clusters. Members of epsilon-Proteobacteria were frequently encountered. Half of these were classified between two known groups, Corre's B and D. The other half of the clones were assigned to new groups, SSSV-BE1 and SSSV-BE2 (Suiyo Seamount sub-vent origin, Bacteria domain, epsilon-Proteobacteria, groups 1 and 2). From this hydrothermal vent field, we detected a novel lineage within the PSB cluster, SSNV-BAI (Suiyo Seamount natural vent origin, Bacteria domain, alpha-Proteobacteria, group 1), which is closely related to Rhodopila globiformis isolated from a hot spring. A number of archaeal clones were also detected from the borehole samples. These clones formed a novel monophyletic clade, SSSV-AE1 (Suiyo Scamount sub-vent origin, Archaea domain, Euryarchaeota, group 1), approximately between methanogenic hyperthermophilic members of Methanococcales and environmental clone members of DHVE Group II. Thus, this hydrothermal vent environment appears to be a noteworthy microbial and genetic resource. It is also noteworthy that some of the findings presented here were made possible by the application of the in situ growth chamber into the hot fluids deep inside the boreholes. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.