Detection of squamous‐cell carcinoma antigen‐expressing tumour cells in blood by reverse transcriptase‐polymerase chain reaction in cancer of the uterine cervix
Detection of squamous‐cell carcinoma antigen‐expressing tumour cells in blood by reverse transcriptase‐polymerase chain reaction in cancer of the uterine cervix
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通过逆转录聚合酶链反应检测宫颈癌血液中表达鳞状细胞癌抗原的肿瘤细胞
DOI：
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发表时间：
1997
影响因子：
6.4
通讯作者：
U. Stenman
中科院分区：
文献类型：
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作者：
J. Stenman;S. Lintula;K. Hotakainen;J. Vartiainen;H. Lehväslaiho;U. Stenman
我们使用鳞状细胞癌（SCC）抗原mRNA的逆转录聚合酶链反应方法检测宫颈癌患者的循环肿瘤细胞。通过细胞加标实验确定，该方法的灵敏度为106个白色血细胞中的10个培养的A431细胞。15例患者中有6例检测到循环肿瘤细胞。在我们的对照组24名女性中，2名足月孕妇检测到SCC抗原mRNA。采样后随访24个月，评估结果。SCC抗原mRNA阳性的6例患者中有3例复发。此外，1例患者发生乳腺癌。在SCC抗原mRNA阴性的9例患者中，有1例复发和1例疾病进展。结果提示，RT-PCR检测外周血SCC抗原mRNA对宫颈表皮样癌的分期和预后评估有一定的临床价值。国际癌症杂志74：75 - 80。© 1997 Wiley利斯公司
We used a reverse transcriptase‐polymerase chain reaction method for squamous‐cell carcinoma (SCC) antigen mRNA to detect circulating tumour cells in patients with carcinoma of the uterine cervix. The sensitivity of the method, as determined by cell spiking experiments, was 10 cultured A431 cells among 106 white blood cells. Circulating tumour cells were detected in 6 of 15 patients. In our control group of 24 women, SCC antigen mRNA was detected in 2 pregnant women at term. We followed up the patients for 24 months after sampling and evaluated the outcome. Three out of 6 patients positive for SCC antigen mRNA have relapsed. Additionally, 1 patient has developed breast cancer. In the group of 9 patients negative for SCC antigen mRNA there has been 1 relapse and 1 case of progression of disease. These results suggest that detection of SCC antigen mRNA in peripheral blood by RT‐PCR could be useful for staging and evaluation of prognosis in epidermoid carcinoma of the uterine cervix. Int. J. Cancer 74:75‐80. © 1997 Wiley‐Liss, Inc.
DOI：
10.1073/pnas.92.8.3147
发表时间：
1995-04-11
影响因子：
11.1
作者：
SCHNEIDER, SS;SCHICK, C;SILVERMAN, GA
通讯作者：
SILVERMAN, GA
人类 40-kD 角蛋白的胚胎表达：来自处理的假基因序列的证据。
影响因子：
9.8
作者：
Savtchenko,ES;Schiff,TA;Jiang,CK;Freedberg,IM;Blumenberg,M
通讯作者：
Blumenberg,M
影响因子：
45.3
作者：
DATTA, YH;ADAMS, PT;ROTH, MS
通讯作者：
ROTH, MS