Co-transport of Na+ and methul-beta-D-thiogalactopyranoside mediated by the melibiose transport system of Escherichia coli.
Co-transport of Na+ and methul-beta-D-thiogalactopyranoside mediated by the melibiose transport system of Escherichia coli.
复制标题
由大肠杆菌的蜜二糖转运系统介导的钠和甲基-β-D-硫代半乳糖苷的共转运。
DOI:
10.1016/0006-291x(77)91663-1
复制
发表时间:
1977
影响因子:
3.1
通讯作者:
T. Wilson
中科院分区:
文献类型:
--
作者:
T. Tsuchiya;J. Raven;T. Wilson
Na+-dependent transport of methyl-β-D-thiogalactopyranoside (TMG) mediated by the melibiose transport system was investigated in Escherichia coli mutants lacking the lactose transport system. When an inwardly-directed electro-chemical potential difference of Na+ was imposed across the membrane of energy depleted cells, transient uptake of TMG was observed. Addition of TMG to cell suspensions under anaerobic conditions caused a transient acidification of the medium. This acidification was observed only in the presence of Na+ or Li+. These results support the idea that TMG is taken up by a mechanism of Na+-TMG co-transport via the melibiose transport system in Escherichia coli.