Insulin and epidermal growth factor stimulate phosphorylation of p185HER-2 in the breast carcinoma cell line, BT474.
Insulin and epidermal growth factor stimulate phosphorylation of p185HER-2 in the breast carcinoma cell line, BT474.
复制标题
胰岛素和表皮生长因子刺激乳腺癌细胞系 BT474 中 p185HER-2 的磷酸化。
DOI：
10.1016/0303-7207(90)90004-r
复制
发表时间：
1990
影响因子：
4.1
通讯作者：
Clinton,GM
中科院分区：
文献类型：
--
作者：
Lin,YZ;Li,SW;Clinton,GM
The product of the HER-2 proto-oncogene, p185HER-2, was found to be amplified approximately 10-fold in the human breast carcinoma cell line, BT474, compared to a cell line, HBL-100, derived from normal breast tissue. To explore the possible role of p185HER-2in growth of the breast carcinoma cells, we investigated factors that may modulate cell growth and phoshorylation of the HER-2 protein product. Two growth factors, epidermal growth factor (EGF) and insulin, stimulated phosphorylation of the HER-2 protein product. In response to insulin, the phosphoserine and phosphothreonine content in p185HER-2was transiently enhanced about 6-fold. When EGF was added to BT474 cells there was 2- to 3-fold enhanced phosphorylation of serine and threonine residues in p185HER-2which was maintained for at least 60 min. Although p185HER-2has been found to be phosphorylated on tyrosine residues following EGF treatment of several different cell types, we estimate that less than 1% of the protein contained phosphotyrosine in the BT474 cells.
