ESTABLISHMENT OF AN IMMATURE MAST-CELL LINE FROM A PATIENT WITH MAST-CELL LEUKEMIA
ESTABLISHMENT OF AN IMMATURE MAST-CELL LINE FROM A PATIENT WITH MAST-CELL LEUKEMIA
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DOI：
10.1016/0145-2126(88)90050-1
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发表时间：
1988-01-01
影响因子：
2.7
通讯作者：
GLEICH, GJ
中科院分区：
文献类型：
--
作者：
BUTTERFIELD, JH;WEILER, D;GLEICH, GJ
从肥大细胞白血病患者的外周血中建立了一个显示出许多未成熟肥大细胞特征的细胞系。培养的细胞含有低水平的组胺，被甲苯胺蓝异染，并含有氯乙酸酯酶，氨基己酸酯酶和类胰蛋白酶活性。这些细胞缺乏T和B淋巴细胞以及髓样细胞标记物，并且不具有IgE受体。异染性阳性细胞的实体瘤已成功地诱导，并在裸鼠中使用5-氮杂胞苷转化细胞连续传代。该细胞系可能有助于肥大细胞及其成分的进一步研究。
A cell line showing many characteristics of immature mast cells has been established from the peripheral blood of a patient with mast cell leukemia. Cultured cells contain low levels of histamine, are stained metachromatically by toluidine blue, and contain chloroacetate esterase, aminocaproate esterase and tryptase activities. The cells lack T and B lymphocyte, as well as myeloid cell markers, and do not possess IgE receptors. Solid tumors of metachromatically positive cells have been successfully induced and serially passed in nude mice using 5-azacytidine transformed cells. This cell line may be useful for future studies of mast cells and their constituents.