Role of calcium in the thyrotropin-releasing hormone-stimulated release of prolactin from pituitary cells in culture.
Role of calcium in the thyrotropin-releasing hormone-stimulated release of prolactin from pituitary cells in culture.
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钙在促甲状腺激素释放激素刺激的垂体细胞释放催乳素中的作用。
DOI：
10.1016/0006-291x(78)91422-5
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发表时间：
1978
影响因子：
3.1
通讯作者：
Donna Maina
中科院分区：
文献类型：
--
作者：
A. H. Tashjian;Marcia E. Lomedico;Donna Maina
促甲状腺激素释放激素（TRH）或50 mM K+刺激急性释放催乳素从GH4 C1株大鼠垂体细胞培养。Ca+2拮抗剂Co+2（2.0 ~ 0.5 mM）和Ca+2螯合剂EGTA（1.0 mM）以剂量相关的方式抑制催乳素的释放。Co+2也减少自发基础催乳素释放。Ca+2（2.0 mM）可部分逆转Co+2（2.0 mM）的抑制作用，而Ca+2（2.0 mM）可完全逆转EGTA（1.0 mM）的抑制作用。在含有0.67至0.74 mM Ca+2的培养基中，由50 mM K+刺激的催乳素的增强释放在10-20分钟时达到最大。Na+（50 mM）没有模拟高K+的效果。我们的结论是Ca+2是介导高K+和TRH对GH4 C1细胞分泌催乳素的作用的一种必需阳离子。
Thyrotropin-releasing hormone (TRH) or 50 mM K+stimulated the acute release of prolactin from the GH4C1strain of rat pituitary cells in culture. The enhanced release of prolactin was inhibited in a dose-related manner by the Ca+2antagonist Co+2(2.0 to 0.5 mM) as well as by the Ca+2chelator EGTA (1.0 mM). Co+2also reduced spontaneous basal prolactin release. There was partial reversal of the inhibitory effect of Co+2(2.0 mM) by Ca+2(2.0 mM) and complete reversal of the inhibitory effect of EGTA (1.0 mM) by Ca+2(2.0 mM). The enhanced release of prolactin stimulated by 50 mM K+was maximal by 10–20 minutes in medium containing 0.67 to 0.74 mM Ca+2. Na+(50 mM) did not mimic the effect of high K+. We conclude that Ca+2is an essential cation in mediating the actions of high external K+and TRH on the release of prolactin by GH4C1cells.