DECLINE IN COLONY-FORMING ABILITY OF MARROW CELLS SUBJECTED TO SERIAL TRANSPLANTATION INTO IRRADIATED MICE
DECLINE IN COLONY-FORMING ABILITY OF MARROW CELLS SUBJECTED TO SERIAL TRANSPLANTATION INTO IRRADIATED MICE
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DOI：
10.1002/jcp.1030640104
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发表时间：
1964-01-01
影响因子：
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通讯作者：
MCCULLOCH, EA
中科院分区：
文献类型：
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作者：
SIMINOVITCH, L;TILL, JE;MCCULLOCH, EA
已知造血祖细胞具有自我更新的能力，因为从这些细胞衍生的克隆含有具有克隆形成能力的细胞。然而，连续移植来自正常骨髓的细胞会导致克隆形成能力的丧失。这一损失反映了集落形成细胞的性质随着传代的变化，因为来自第一代的汇集集落的细胞具有集落形成能力，而这种集落形成能力在第二代时丧失。事实上，当单个克隆的细胞被连续移植时，克隆形成能力就会丧失。结论：正常小鼠骨髓集落形成细胞的增殖能力虽然广泛，但并不是无限的。
Hemopoietic colony-forming cells are known to be capable of self-renewal, since colonies derived from these cells contain cells with colony-forming ability. However, serial transplantation of cells derived from normal marrow results in a loss of colony-forming ability. This loss reflects a change in the properties of colony-forming cells with passage since cells derived from pooled colonies at the first passage had colony-forming capacity, which was lost at the second passage. Indeed, when cells from individual clones were transplanted serially, colony-forming ability was lost. It is concluded that the proliferative capacity of normal mouse marrow colony-forming cells, while extensive, is not unlimited.